[Histonet] Re: Aqueous mountant suggestions please

From:"Andrea Hooper"

We don't heat Faramount and it works well for our chromagens such as 
AEC+.  And like Gayle's lab, for DAB+ frozens we dehydrate and clear 
and get beautiful, permanent results. Highly recommended.


At 10:34 AM -0600 4/24/07, Gayle Callis wrote:
>The Faramount works, but it must be heated before coverglass 
>application, and the heating adds another step you have to deal 
>with, Howevere, it is very nice aqueous media that sets up hard and 
>We have also done frozen sections with DAB and set up the 
>dehydration/clearing for permanent mounting media.  I must admit 
>this gives a superb final preparation that when dry, the mounting 
>media doesn't retract under the coverglass.  It is not that 
>difficult to set up the dehyration/clearing/permanent mountant,  and 
>there are some nice mini-staining devices to help you out depending 
>on how many slides you stain at one time.   We prefer 
>dehydration/clearing/and permanent media with DAB.
>At 09:39 AM 4/24/2007, you wrote:
>>Quick query,
>>I need and aqueous mountant for immunohistochemistry slides. I am
>>currently using Vectamount AQ from Vectashield but am having some
>>problems with it not hardening completely and it doesnt seem to last
>>very long ie. the use by date is already up and I'm only halfway through
>>the bottle.
>>Mostly I am mounting immunolabelled frozen tissue sections with DAB. I
>>know aqueous mountants are generally used for AEC but I dont see the
>>point in doing the alcohol and xylene steps if an aqueous mountant
>>works. I dont do paraffin sections so dont already have this set-up.
>>Vector say that their aqueous mountant is also suitable for DAB but is
>>this true for all aqueous mountants? I've been looking at Faramount
>>Aqueous mountant from Dako - any comments?


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