Hello Bruce,

1st the glut fixed tissue is probably not good for ISH or IHC. You can use them to maybe practice cutting.

I have done drosophila for IHC by fixing in an alcoholic formalin. The alcohol helps with faster penetration and better fixation.

When processed to paraffin, face off the block, soak in ammonia water about 5-10 minutes, move knife to new edge, chill block, and cut.

 
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc. 
630 N. Broadway 
Moore, OK 73160 
405-759-3953 
contact@excaliburpathology.com
www.excaliburpathology.com
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