[Histonet] Colonic Lymph Nodes

From:"Hannen, Valerie"

Happy Lab Week to all,
  I am responding to Wanda's request for suggestions for finding colonic
nodes after fixation.
While we do not use 10% NBF(we use Zinc Formalin), we do use a product
after fixation. 
This is solution is called "O-FIX" and we buy it from Surgipath. It does
a wonderul job of 
enhancing the nodes so that they can be easily found. If enhancing is
not time critical,
we place the peri-colonic / mesentaric fat in the "O-FIX" overnight. If
time is critical, we place
the fat in the "O-FIX" and heat it at 42-43 degrees centigrade for
approximately 3 hours.
                   I hope this will help.
          Valerie Hannen, MLT(ASCP),HTL,SU(FL)
           Parrish Medical Center
           Titusville,Florida 32796
           (321) 268-6333 Ext. 7506


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