I was wondering if anyone would be so kind as giving input on the different
ways of preparing a cell button.  We work a lot with Pleural Fluid and
Ascites Fluid.  I have been told two different ways.  One is just 1/2 and
1/2 with fluid and 95%alcohol and spend down decant and get your cell
button.  The other is spend down the fluid decant add 10%formalin respin and
let set then get your cell button.  Of course this all after I have made my
cytospins.  We do not have a ThinPrep type machine.  Any input would be very
appreciative.  I realize there is probably more ways to do this that I have
not heard of.
Cheers,
 
Karen Heckford HT (ASCP) CE
Lead Histology Technician
Histology/Pathology Department
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
Fax: 415-750-8123
email: kheckfor@chw.edu