Albert,
Can you tell me how to do the "block well" before
implementing the second (rabbit primary#2) set Ab
staining?
Thanks in advance!
Jimmy
--- AGrobe2555@aol.com wrote:
> Yves,
> Just do the complete stain for one (block, rabbit
> primary#1, fluorescent
> secondary#1), block well and then do the second
> (rabbit primary#2, fluorescent
> secondary#2). Use two different color anti-rabbit
> fluorophores. I have done
> this in the past with no apparent problems.
>
> Another option is to pre-react the two primaries
> with different anti-rabbit
> fluorophores in separate tubes, and then add to the
> section.
>
> Albert
>
> Albert C. Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue Engineering Lab, Saint Patrick Hospital
>
>
>
>
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