Re: [Histonet] derm path

Thank you for your input.  I have an extensive knowledge of IHC and am familiar with all of the concerns that you mentioned in your email.  I do IHC and FISH everyday, I was just questioning what other antibodies could they ask for, in addition to the more common ones.  I asked this question not because of inexperience, but because I have been out of the hospital setting for a few years and currently the specimens I receive are GI and GU.
Roxanne Soto HT(ASCP)QIHC
Lab Supervisor
Physicians RightPath
Tampa, Fl
-----Original Message-----
Sent: Mon, 16 Apr 2007 10:30 AM
Subject: Re: [Histonet] derm path

I think the more important issues here are 1.) how much experience do you have 
doing immunohistochemical (IHC) staining and 2.) what will be your volume as you 
move forward.  Keep in mind that many DermPath diagnoses are based on IHC 
findings and you need to perform validations on-site (for every primary 
antibody) to demonstrate that you are obtaining the correct IHC results.  Are 
you prepared to deal with issues concerning fixation and tissue processing, 
antigen retrieval, nonspecific staining, antibody cross-reactivity, appropriate 
negative and positive controls, etc.?  In my opinion, you should not be doing 
IHC unless you are performing the tests on a regular basis and you have an 
experienced staff  to do the job right, including a pathologist to assist and 
support you.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>>  04/16/07 9:35 AM >>>
Hello all,
We are thinking of adding another client, but it is a derm client.  Can anybody 
tell me what all antidoies I will need to have on hand?  I know the basics: 
Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? 
Thanks in advance,
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