Re: [Histonet] derm path

From:"Richard Cartun"

I think the more important issues here are 1.) how much experience do you have doing immunohistochemical (IHC) staining and 2.) what will be your volume as you move forward.  Keep in mind that many DermPath diagnoses are based on IHC findings and you need to perform validations on-site (for every primary antibody) to demonstrate that you are obtaining the correct IHC results.  Are you prepared to deal with issues concerning fixation and tissue processing, antigen retrieval, nonspecific staining, antibody cross-reactivity, appropriate negative and positive controls, etc.?  In my opinion, you should not be doing IHC unless you are performing the tests on a regular basis and you have an experienced staff  to do the job right, including a pathologist to assist and support you.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>>  04/16/07 9:35 AM >>>
Hello all,
We are thinking of adding another client, but it is a derm client.  Can anybody tell me what all antidoies I will need to have on hand?  I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? 
Thanks in advance,
AOL now offers free email to everyone.  Find out more about what's free from AOL at
Histonet mailing list

Confidentiality Notice

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged.  Any unauthorized review, use, disclosure, or distribution is prohibited.  If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message.

Histonet mailing list

<< Previous Message | Next Message >>