Hi Helen:

    Bouin's fixed material is usually very easy to cut. Depending on how 
large your specimens are 15 min  per station may not be enough.

Geoff

Helen E Johnson wrote:

>Dear Histonetters,
>           Has anyone experienced chatter artifact while sectioning Bouins
>fixed mouse tissue?  The tissue is fixed in Bouins fixative for 24 hrs. and
>transferred to 70% EtOH for rinsing.  The tissues are processed 15 min. at
>each solution station.
>Helen Johnson    (hej01@health.state.ny.us.)
>
>
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>
>  
>



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