Re: [Histonet] bouins fixed mouse tissue

From:Andrea Grantham



helen,
What kind of tissue are you trying to cut?
I frequently get mouse ovaries fixed for 24 hrs in Bouins and transfered to 
70% ETOH. They usually cut fine but in your case my suggestion is SOAK, 
SOAK, SOAK in icy water.
Not long ago someone here suggested putting a little glycerin in the 
soaking water - you can find the actual posts on the archives - but I tried 
it and was delighted with the results.

Andi Grantham


At 02:07 PM 4/9/2007 -0400, Helen E Johnson wrote:

>Dear Histonetters,
>            Has anyone experienced chatter artifact while sectioning Bouins
>fixed mouse tissue?  The tissue is fixed in Bouins fixative for 24 hrs. and
>transferred to 70% EtOH for rinsing.  The tissues are processed 15 min. at
>each solution station.
>Helen Johnson    (hej01@health.state.ny.us.)
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>