Re: [Histonet] Need a Fixative Formula

From:"Bryan Hewlett"



Cheri,

Just what is the published reference for this wonderful elixir???

Bryan

----- Original Message ----- 
From: "Cheri Miller" 
To: "'Pamela Marcum'" ; 

Sent: Friday, April 13, 2007 2:32 PM
Subject: RE: [Histonet] Need a Fixative Formula


>
> This is our lilies we use it for our fatty tissues and love it.
>
>
> Stock solution;   200 ml glacial acetic acid
>                  800 ml 95% alcohol
>
> Working solution; 1 part stock to 3 parts 10% neutral buffered formalin.
>
> Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela
> Marcum
> Sent: Friday, April 13, 2007 10:24 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Need a Fixative Formula
>
>
>
> Does anyone have a formula for the old original Lillie's
> Fixative?  Someone asked me for it and I can't find it in any of my
> books here.  Thanks in Advance!!
>
> Best Regards,
>
> Pamela A Marcum
> Manager, Histology Special Procedures
> University of Pennsylvania
> School of Veterinary Medicine
> R.S. Reynolds Jr.  CORL
> New Bolton Center
> 382 West Street Road
> Kennett Square, PA 19348
>
> Phone - 610-925-6278
> Fax     - 610-925-8120
> E-mail - pmarcum@vet.upenn.edu
>
>
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>
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