Re: [Histonet] McMannas PAS Light Green

From:"Joe Nocito"

we perform a PAS/Fungus on all toenails we gross (hence Joe the Toe). The 
doctors I work for like out light green strong. I can't count how many times 
they kicked back trays of slides that weren't stained dark enough. We have a 
TissueTek stain line that we use for our special stains. We have an extra 
container filled with 95% ETOH we use to rinse off the Fast Green. Every 
time we did a quick rinse in water, the green always washed out. Personally, 
the magenta color with the dark green just curls my toes up. I'm thinking of 
painting my living room with those colors.


----- Original Message ----- 
Sent: Monday, April 09, 2007 8:50 AM
Subject: [Histonet] McMannas PAS Light Green

> Morning Histonetters,
> I am in need of everyones help. I am doing a McMannas PAS with light green 
> on a fungus but am un-sure how much the light green it to show. Every time 
> I have done this stain so far the light green has been extremly faint.
> I have been using a fungus in lung control.
> I have also been unable to find a photo of what this stain should look 
> like.
> Thanks for all of your Help.
> Matt Lunetta
> Longmont United Hospital
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