Hi Jamie:
I think I agree with Rene, not enough fixation. I suggest changing
fixative to a formalin-alcohol-acetic mixture. It will fix much faster
and give excellent preservation of glycogen.
It frightens me to think that turn around time is more important
than good results.
Geoff
Jamie E Erickson wrote:
>Hi All,
> I hope someone can help with this problem. I am working on a
>toxicology study that requires sections of Heart, liver and Lung from rats
>on study . These studies we want a quick turn around so we get the tissues
>from necropsy in the afternoon, livers are scored at necropsy and put
>into formalin jars. I gross the samples Liver (left, midial, caudal lobes
>into one cassette) the next morning done by 11am (liver is still pink in
>the middle). The samples are put on the processor (11 hour processor run)
>that night with formalin in the first station so fixation after grossing
>is 10 hours. The next day I embed and section the samples and dry the
>sections overnight. Slides are stained and given to the pathologist that
>day.
>
>My problem is the liver samples all other blocks are fine, the liver
>blocks section fine but on drying either in a rotating drying oven
>vertically or on the bench at room temp overnight some NOT all of the
>liver samples have lots of cracks and some fall off. It very much looks
>like areas of water as it dried causes these cracks. Anyone have ideas to
>remedy this problem..?? They want to keep this turn around time if
>possible and liver is a key read out... Any ideas...
>
>Thanks
>
>Jamie
>_______________________________
>Jamie Erickson
>Sr. Research Associate
>Department: DSMP
>Abbott Bioresearch Center
>100 Research Drive
>Worcester, MA 01605-4341
>508-688-3134
>FAX: 508-793-4895
>e-mail: jamie.erickson@abbott.com
>
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>
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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