Re: [Histonet] HSV 1&2 staining

From:James L Burchette

Mr. Robinson:
I prepare an antibody cocktail of HSV I & II. No pretreatment for FFPE 
tissues. 30-45 minute primary, 20 minute HRP labeled Envision Plus, 
standard DAB (DAB+ not needed). HSV will work just as well with a 
biotin/streptavidin system. Rather straight forward, no tricks or hoops to 
jump through.

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina" 
Sent by:
04/13/2007 10:40 AM


[Histonet] HSV 1&2 staining

Could someone share the current methodology that they are using on the 
DAKO Autostainer Plus for HSV 1 & 2 staining ?  Thanks.

Jim Robinson, M.S., HTL/HT (ASCP)
Director of Laboratory Operations
Orizon Diagnostics, LLC
102 Chestnut Avenue
Westmont, Illinois 60559
630-321-1506 (fax)

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