Re: [Histonet] Demonstration of H. Pylori

From:James L Burchette

Hello Delores and everyone else,

We use IHC for the demonstration of H pylori. I don't get caught up in the 
argument of IHC vs. special stains. If our doctors like IHC, that's fine 
with me. Job security says it all.
I use Dako's rabbit polyclonal antibody at 1:100, pretreat with Brigati's 
stable pepsin, and detect with a biotin/streptavidin system. I like AEC, 
but sometimes use DAB as the chromogen.

After taking a couple years off from Histonet.... I'm back

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"

"Dolores Townsend"  
Sent by:
04/13/2007 08:45 AM


[Histonet] Demonstration of H. Pylori

Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
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