RE: [Histonet] ready to use antibody

From:"Ingles Claire"

I have been playing on getting some immunos up and running for our Mohs lab (so frozen skin sample only). I have found the Biocare kits work great. I use the Alk. Phos. kit with a fast red chromogen (permanent, not aqueous) I have tried the aqueous fast red Alk. Phos. kit from Innovex and it doesn't work for me. I've tried everything I can think of. The kit from Biocare worked the first time I tried it. They also have great customer service with tons of tech support. Hope this helps. I know how frustrating this stuff can be. Good Luck.
Claire Ingles
UW Hospital and Clinics
Madison WI


From: on behalf of Kalleberg, Kristopher
Sent: Fri 4/6/2007 1:21 PM
Subject: [Histonet] ready to use antibody

Has anyone ever used the ready to use antibodies which are prediluted?
They are offerred by Lab Vision/Neomarkers.  Is there any special
protocol for these antibodies?  I have run an experiment twice with this
prediluted antibody and have had no results.  Technical support was
unable to help me with these items.Any help or information will be
greatly appreciated.  Thank you.

Kris Kalleberg  
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