RE: [Histonet] Zinc Formalin and Iron stains

From:Gayle Callis

We have used zinc formalin, although it was Anatech's buffered zinc 
formalin formulation, and had wonderful results on human kidney biopsies - 
excellent morphology and no exploded cells that the pathologist could 
see.  He loved the results.   The sections cut at 1 - 2 um.  When we tried 
this fixative on animal tissues, we also had great success.  Could it be it 
was a zinc formalin that was NOT buffered correctly or even at all that 
caused the exploding cells?

As for iron staining after zinc formalin, not sure what the results are. 
However, you should speak to Anatech, in particular, Ada Feldman, she could 
let you know if iron staining is compromised by this fixative.

At 12:32 PM 4/16/2007, you wrote:
>I don't know, we only use 10% NBF for bone marrows here, but I've seen a
>video of some cells getting fixed with zinc formalin.  The cells were
>popping and exploding all over the place.  Made me think, "no wonder you
>get that great nuclear detail."

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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