RE: [Histonet] When staining IHC's, are labs putting controltissue on every slide?

From:"Tom McNemar"



We put a positive control on every slide.  We use the slides with the red box for most of them.  The only ihc controls that we cut ahead are the H. Pylori.  everything else we cut as needed.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard
Cartun
Sent: Wednesday, April 11, 2007 6:12 PM
To: histonet@lists.utsouthwestern.edu; James F. Happel
Subject: Re: [Histonet] When staining IHC's, are labs putting
controltissue on every slide?


No, our positive control tissues are placed on separate slides.  We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Happel, James F."  04/10/07 4:29 PM >>>
Good Afternoon All,

When staining IHC's, is anyone/everyone putting control tissue on every slide?
Both + and - ?

James

-----Original Message-----
From: Liz Beitman [mailto:LBeitman@cellmarque.com] 
Sent: Friday, March 23, 2007 12:19 PM
To: Happel, James F.
Subject: RE: [Histonet] Question about historical photographs

Hi James,

I just want to mention that we do have some posters of all the paintings
that adorn our catalog that I can send to you.  If you take a look in
our catalog (volume 7), page 79, it will give you an idea of some of the
posters.  Some are rather interesting from different points of view of
featured artists (Picasso, Klimt, and Michelangelo).  They might fit in
with your history presentation.  If you find any of interest, I would
look into either getting you an image or the actually poster.  Please
let me know.

Thanks,

Liz Beitman
Cell Marque
1-800-665-7284 x 8981
lbeitman@cellmarque.com 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Friday, March 23, 2007 7:49 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Question about historical photographs

Good Day,

I am putting together a presentation on the past, present and future of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital





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