Marcia,
I think you need to be more specific.  Are the "train rails" showing up
when you cut a ribbon?  Is it only in GI biopsies? Is it a new and
different batch of paraffin?  Have you tried stirring the paraffin a bit
before embedding?  If it is during sectioning that these train rails are
appearing then the problem is probably in your paraffin.  
Please don't send the snow my way.  I'm sick of it.  Since I'm in the
basement and don't have any windows I don't know if there is any sun to
send your way but I'll go check.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek@digestivespecialists.com

 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia
Funk
Sent: Tuesday, April 10, 2007 10:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI BX


Histo Folks,

This past week we have noticed that our GI Biopsies look like a train
rails running through.  Knives are sharp
and techs that are sectioning have been in the field for 10 years plus.
We do not process the GI's in a shorter
processing, maybe I should.  What are your thoughts.  Also we are using
PARAPLAST Plus Medium for both
processing and embedding.  Do most labs use different paraffin for
embedding ?  We have the Sakura
VIP Tissue processors.   I would appreciate any comments that you might
share.

If you have sunshine send it my way as we are to get 4 to 8 inches on
new snow today and tomorrow.

Marcia



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