Why would a negative control have more and darker staining than the actual
test slides with the antibody? I am used to adjusting dilutions when both are
too high, but what to do when lowering a dilution to get rid of background,
gets rid of what little staining you have? I am using avidin/biotin blocks in
addition to the normal serum block. Could it be something to do with the
tissue? I am having this problem with rat liver, though I never have it with
other rat tissues. It is a PCNA stain I am already running it at 1:10,000,
which is way less than what I run it on any other tissue.
Renee' Till, HT
Arkansas Children's Nutrition Center
1212 Marshall St./N2021
Little Rock, AR 72202
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