[Histonet] Re: Bouins fixed mouse tissue


I agree with Andi. My lab mainly uses Bouin's for adult mouse testes with  
excellent results. We fix 8 to 24 hours in Bouin's, rinse several times  
throughout the day with water, then 2 days in 50% ETOH. Then the processing  schedule 
is 30 minutes each station. Sometimes I go up to 1 hr each station if I  have 
a lot of other tissue that needs longer processing. Both schedules have  
produced excellent results in our lab and the faced blocks need only a  few 
minutes of wet ice soaking for beautiful sections. I still have not gotten  very 
good results with formalin fixed adult mouse testes. The formalin blocks  look 
good, cut good, but morphologically look bad. So I stick with the  Bouins.
Donna Emge, HT(ASCP)
Northwestern University
303 E. Superior, Lurie 7-220
Chicago, IL 60611
_d-emge@northwestern.edu_ (mailto:d-emge@northwestern.edu) 

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