[Histonet] Job Opening

From:"Jen, Shirley"



Research Associate position available at Roche Palo Alto, California.
This position will function as a histotechnologist in the Non-Clincial Drug Safety group, performing histology, from wet tissue trimming to staining, immunohistochemistry, and imaging tasks to support research and investigative toxicology studies.  Please send resume to 
shirley.jen@roche.com. 

Shirley Jen
Research Supervisor, Histology
Pathology Department
Roche Palo Alto LLC
3431 Hillview Avenue
Palo Alto, CA 94304
Phone: 650-852-1810
Fax: 650-852-1098
e-mail: shirley.jen@roche.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, April 11, 2007 3:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 41, Issue 17

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Today's Topics:

   1. FW: HTL question (Bartlett, Jeanine (CDC/CCID/NCZVED))
   2. Re: In situ hybridization on insects: fixing, processing	&
      cuticle softening advice (Bruce Webber)
   3. Re: FW: HTL question (Dolores Townsend)
   4. HTL Question (Judy Collins)
   5. Simple device for holding bones (Linda Jenkins)
   6. Re: In situ hybridization on insects: fixing, processing	&
      cuticle softening advice (Bruce Webber)
   7. RE: FW: HTL question (Liz Chlipala)
   8. RE: FW: HTL question (Bartlett, Jeanine (CDC/CCID/NCZVED))
   9. RE: FW: HTL question (Monfils, Paul)
  10. RE: Re: Bone saw (Ingles Claire)
  11. RE: Re: Bone saw (Philip Oshel)
  12. RE: Re: Bone saw (Akemi Allison-Tacha)
  13. RE: FW: HTL question (Horn, Hazel V)
  14. RE: Re: Bone saw (Philip Oshel)
  15. RE: FW: HTL question Can diagnose fungus from H&E
      (Akemi Allison-Tacha)
  16. RE: FW: HTL question Can diagnose fungus from H&E (Horn, Hazel V)
  17. Seasonal snap freezing noses and ears in Montana  (Gayle Callis)
  18. web resource for histology (Philip Oshel)
  19. RE: Re: Bone saw (Rittman, Barry R)
  20. RE: web resource for histology (Horn, Hazel V)
  21. Re: When staining IHC's, are labs putting control	tissue on
      every slide? (Richard Cartun)
  22. RE: FW: HTL question (Bartlett, Jeanine (CDC/CCID/NCZVED))
  23. Re: FW: HTL question (Richard Cartun)


----------------------------------------------------------------------

Message: 1
Date: Wed, 11 Apr 2007 13:23:30 -0400
From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" 
Subject: [Histonet] FW: HTL question
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

All,

I am forwarding this email from a young lady I know that is studying for
her HTL.  I will pass along your responses.  Thanks in advance for
helping.

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov



> ______________________________________________ 
> From: 	Packard, Michelle (CDC/CCID/NCZVED)  
> Sent:	Wednesday, April 11, 2007 1:21 PM
> To:	Bartlett, Jeanine (CDC/CCID/NCZVED)
> Subject:	RE: HTL question
> 
> The following question is in the Histotechnology BOR Study Guide
> Practice Questions 2nd Edition published by ASCP:
> 
> For light microscopic evaluation it is generally necessary to use
> special stains to demonstrate fungi in tissue sections because fungi:
> A. can only be seen using silver impregnation
> B. are removed in the routine staining process
> C. stain variably with the H&E procedure
> D. are never demonstrated with routine procedures
> 
> The consensus here is that C is the best answer, however, the answer
> key lists D as correct.  Would like to get some feedback on this if
> possible.  Is the answer key wrong or am I missing something?  
> 
> In preparing for the exam, has anyone run across any other answers
> from this text that appear to be wrong?  


------------------------------

Message: 2
Date: Wed, 11 Apr 2007 18:25:50 +0100
From: Bruce Webber 
Subject: Re: [Histonet] In situ hybridization on insects: fixing,
	processing	& cuticle softening advice
To: Histonet 
Message-ID: <461D1A1E.7020709@cefe.cnrs.fr>
Content-Type: text/plain; charset=ISO-8859-1

Perhaps I shouldn't have sent my request for information so close to the
Easter holidays....  I'm yet to receive any feedback on my query email
of April 5th, so if anyone has some input on any of the issues I would
greatly appreciate it!

With thanks in advance, Bruce



------------------------------

Message: 3
Date: Wed, 11 Apr 2007 13:35:10 -0400
From: "Dolores Townsend" 
Subject: Re: [Histonet] FW: HTL question
To: "Jeanine (CDC/CCID/NCZVED) Bartlett" ,
	histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

As for a lot of tests, the answer is a bit tricky and confusing, but D
is right: although the pathologist can see something that MIGHT be
fungus on the routine stain, (s)he will ask for a special stain (GMS or
other) to confirm the presence of fungus. Hence, D. Fungi are never
demonstrated with routine procedure. You will need the special stain to
be sure. This is true for most special stains: the pathologist sees
something on the routine stain, but it is not conclusive, so special
stains are needed to confirm the diagnostic.
Dolores


------------------------------

Message: 4
Date: Wed, 11 Apr 2007 13:36:07 -0400
From: "Judy Collins" 
Subject: [Histonet] HTL Question
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Although my first thought was that "C" was correct, on second thought, I
think it probably is "D" as they stain the same color as the background
(counterstain) and therefore tend to blend in.

Judy



------------------------------

Message: 5
Date: Wed, 11 Apr 2007 13:40:17 -0400
From: Linda Jenkins 
Subject: [Histonet] Simple device for holding bones
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<5.2.1.1.2.20070411132713.01f43c98@mailhost.ces.clemson.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Terry,
         You stated:
         "PS The recurring problem of sawing bones drives me nuts. The 
sawing bit is easy, you just need a saw, it's the holding of the bone 
that's the problem."

         Next time you are out shopping, pick up a melon ball device in 
assorted sizes.  They are perfect for holding a femoral head to guide into 
the table top band saw or while using the Stryker Saw.  They are also great 
for holding irregular shaped bones such as clavicles, etc.

         From the kitchen to the lab... :-)
         Linda

PS: Now, if we could just figure out how to put those nifty stir bars into 
our home cook pots so we would not have to stand and stir...hmmm!


Linda Jenkins, HT
Clemson University
Dept. of Bioengineering
Clemson, SC 29634-0905
864.656.5553
http://www.ces.clemson.edu/bio/research/histo.htm




------------------------------

Message: 6
Date: Wed, 11 Apr 2007 18:40:06 +0100
From: Bruce Webber 
Subject: Re: [Histonet] In situ hybridization on insects: fixing,
	processing	& cuticle softening advice
To: Histonet 
Message-ID: <461D1D76.8050409@cefe.cnrs.fr>
Content-Type: text/plain; charset=ISO-8859-1

... and here is the original email, given that the previous email does
not seem to link up to the original message thread.

On 05/04/2007 16:38, Bruce Webber wrote:
> Hi Histonetters,
>
> I am looking for advice on preparing and processing small (c. 2-3mm
> long) adult ants (i.e. with a well-formed chitinous exoskeleton) for
> in situ hybridization (ISH) detection of bacteria (using DNA probes to
> target RNA).  The ants are transverse sectioned into 3 parts before
> fixing to allow greater penetration into each of the 3 main body cavities.
>
> I've trawled the archives for various options and have consulted my
> histological mentor, Bruce Abaloz, but after not finding what I was
> looking for, I would greatly appreciate advice, opinions and
> recommendations from others.  I do promise to post a summary of any
> responses together with any methodological tips I found along the way!
>
> The main problem I see is dealing with the cuticle to allow for good
> sectioning (5-8um), but using chemicals & methods that don't interfere
> with ISH, damage the target RNA, or interfere with the efficiency of
> the ISH probes.  My understanding is that the following factors need
> to be addressed:
>
> 1) Fixing the tissue: 
> The only available material available at this stage was fixed in 2%
> gluteraldehyde (12h at 4C) and then stored (> 3 weeks) in 80% EtOH
> (meaning that gut excision is not an option due to brittle material). 
> In the future, 4% paraformaldehyde, 10% neutral buffered formalin or
> just straight into 70% EtOH (with slightly shorter storage times)
> could also be considered as options if people feel that they may be
> better (the latter option would certainly increase the availability of
> material).
>
> 2) Softening the cuticle: 
> IMHO this is the biggest potential area for chemical
> damage/interference with ISH.  Proposals I am aware of that would not
> be compatible with ISH due to acidic damage of the RNA are [1]
> Perenyi's fluid (4:3:3, 10% nitric acid:100% EtOH:0.05% chromic
> acid);  [2] Diaphanol (50% glacial acetic acid saturated with ClO2);
> and [3] Bouin's fixative (3:1:2, saturated picric
> acid:formaldehyde:glacial acetic acid).
>
> However, other suggestions from previous posts which I know very
> little about include [4] chloral hydrate processing (after tissue
> dehydration, melt equal parts of chloral hydrate & phenol and immerse
> tissue for up to 7 days, followed by chloroform as an intermediary
> agent);  [5] Mollifex Gurr (glycerol, phenol, acetone and EtOH
> solution, applied to the cutting surface of the paraffin block);  [6]
> Nair (as in the hair removal cream - thioglycolate salts & calcium
> hydroxide, between fixing and processing, soak the tissue for c.
> 24hrs); and [7] Phenol (after fixation, soak fixed specimens in 4%
> phenol (in 80% EtOH) for 24 hours).  Lastly, there is 1% DMSO (added
> to the fixative), but given that the ant is chopped into 3 sections,
> this may not be necessary to ensure adequate penetration (but does
> DMSO also improve sectioning?).
>
> 3) Processing the tissue: 
> The ongoing debate of xylene v histoclear (or histosolve)..... 
> Histoclear is probably safer, but does xylene produce better results? 
> Has anyone had experience with either on similar invertebrate
> material?  Others have suggested using isopropyl alcohol (instead of
> EtOH) for dehydration as is tends to have a less hardening effect on
> the tissue.
>
> 4) Material for embedding: 
> I've seen it argued that tougher material such as that containing
> chitinous exoskeletons benefits from using a harder embedding medium
> (e.g. TissuePrep 2 from Fisher) or one with better infiltration (e.g.
> Paraplast X-tra) over standard paraffin.  I've also read that
> Paraplast Plus (containing DMSO) makes sectioning of cuticles easier. 
> Any opinions/recommendations/experience with similar material and any
> of these embedding mediums would be appreciated!
>
> Thank you all in advance for your help with this matter (I apologise
> for the length of this post!).
>
> Bruce Webber
> Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, France
>
> (Key words: cuticle, exoskeleton, chitin, insect, invertebrate, ant,
> in situ hybridization, ISH, softening.)



------------------------------

Message: 7
Date: Wed, 11 Apr 2007 12:06:48 -0600
From: "Liz Chlipala" 
Subject: RE: [Histonet] FW: HTL question
To: "'Dolores Townsend'" ,
	
Message-ID: <000201c77c64$2c5d8a10$0d00a8c0@domain.Premier>
Content-Type: text/plain;	charset="us-ascii"

ASCP study guide

Some of the questions that are in the HT/HTL study from ASCP are questions
that used to be on the exam but were removed because they did not perform
well for a variety of reasons. They did not differentiate between
knowledgeable candidates and others that did not know as much, or perhaps
there was more than one correct answer, but they still give the candidate an
idea of how the questions are written, etc. This question was probably
removed from the exam and is in the booklet for the same reason it is under
discussion now.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: Wednesday, April 11, 2007 11:35 AM
To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] FW: HTL question

As for a lot of tests, the answer is a bit tricky and confusing, but D
is right: although the pathologist can see something that MIGHT be
fungus on the routine stain, (s)he will ask for a special stain (GMS or
other) to confirm the presence of fungus. Hence, D. Fungi are never
demonstrated with routine procedure. You will need the special stain to
be sure. This is true for most special stains: the pathologist sees
something on the routine stain, but it is not conclusive, so special
stains are needed to confirm the diagnostic.
Dolores
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


__________ NOD32 1971 (20070110) Information __________

This message was checked by NOD32 antivirus system.
http://www.eset.com





------------------------------

Message: 8
Date: Wed, 11 Apr 2007 13:55:20 -0400
From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" 
Subject: RE: [Histonet] FW: HTL question
To: "Liz Chlipala" ,	"Dolores Townsend"
	,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Good point.  It definitely seems like a poorly designed question. 


Jeanine Bartlett
Infectious Disease Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Wednesday, April 11, 2007 2:07 PM
To: 'Dolores Townsend'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] FW: HTL question

ASCP study guide

Some of the questions that are in the HT/HTL study from ASCP are
questions that used to be on the exam but were removed because they did
not perform well for a variety of reasons. They did not differentiate
between knowledgeable candidates and others that did not know as much,
or perhaps there was more than one correct answer, but they still give
the candidate an idea of how the questions are written, etc. This
question was probably removed from the exam and is in the booklet for
the same reason it is under discussion now.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540
liz@premierlab.com www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: Wednesday, April 11, 2007 11:35 AM
To: Jeanine (CDC/CCID/NCZVED) Bartlett;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] FW: HTL question

As for a lot of tests, the answer is a bit tricky and confusing, but D
is right: although the pathologist can see something that MIGHT be
fungus on the routine stain, (s)he will ask for a special stain (GMS or
other) to confirm the presence of fungus. Hence, D. Fungi are never
demonstrated with routine procedure. You will need the special stain to
be sure. This is true for most special stains: the pathologist sees
something on the routine stain, but it is not conclusive, so special
stains are needed to confirm the diagnostic.
Dolores
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


__________ NOD32 1971 (20070110) Information __________

This message was checked by NOD32 antivirus system.
http://www.eset.com



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 9
Date: Wed, 11 Apr 2007 14:21:43 -0400
From: "Monfils, Paul" 
Subject: RE: [Histonet] FW: HTL question
To: 
Message-ID:
	<4EBFF65383B74D49995298C4976D1D5E273C66@LSRIEXCH1.lsmaster.lifespan.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

It's a poor question because the last choice is ambiguous. When  you read "are never demonstrated" you naturally take that to mean "cannot be seen". However, we know that fungi can in fact be seen in an H&E section. Therefore they are "demonstrated" to some degree. But they are not specifically demonstrated in a way that makes them obvious against the background.




------------------------------

Message: 10
Date: Wed, 11 Apr 2007 13:29:11 -0500
From: "Ingles Claire" 
Subject: RE: [Histonet] Re: Bone saw
To: 
Message-ID:
	<08A0A863637F1349BBFD83A96B27A50A120021@uwhis-xchng3.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="iso-8859-1"

 

 

I know what you mean!  Here we can't get a tissue processor because the
Breast Radiology folks "forgot" to order all of the required components
for their digital scanner.  Again, millions versus thousands. 


 Maybe we all need to hire publicity consultants or agents to get the word out... :)

 

Claire Ingles

UW Hospital

Madison WI




------------------------------

Message: 11
Date: Wed, 11 Apr 2007 14:48:09 -0400
From: Philip Oshel 
Subject: RE: [Histonet] Re: Bone saw
To: Histonet@Pathology.swmed.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Rubber bracelets, H&E colored.
Enjoy the snow today! 2 years ago, I would've been on the 10th floor 
of Animal Sciences, and I suspect unable to see the UW Hospital for 
the flakes.

Phll

>
>
>
>
>I know what you mean!  Here we can't get a tissue processor because the
>Breast Radiology folks "forgot" to order all of the required components
>for their digital scanner.  Again, millions versus thousands.
>
>
>  Maybe we all need to hire publicity consultants or agents to get 
>the word out... :)
>
>
>
>Claire Ingles
>
>UW Hospital
>
>Madison WI
-- 
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
voice: (989) 774-3576
dept. fax: (989) 774-3462



------------------------------

Message: 12
Date: Wed, 11 Apr 2007 12:00:20 -0700 (PDT)
From: Akemi Allison-Tacha 
Subject: RE: [Histonet] Re: Bone saw
To: Philip Oshel , Histonet@Pathology.swmed.edu
Message-ID: <912935.16875.qm@web31302.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Yeah, Snow Brizzard in the middle of April...After
coming back from 75 degree weather.  I feel like like
Dorothy clicking her heels.... THERE'S NO PLACE LIKE
HOME!! THERE'S NO PLACE LIKE HOME!!  Won't be long
now.
Akemi
--- Philip Oshel  wrote:

> Rubber bracelets, H&E colored.
> Enjoy the snow today! 2 years ago, I would've been
> on the 10th floor 
> of Animal Sciences, and I suspect unable to see the
> UW Hospital for 
> the flakes.
> 
> Phll
> 
> >
> >
> >
> >
> >I know what you mean!  Here we can't get a tissue
> processor because the
> >Breast Radiology folks "forgot" to order all of the
> required components
> >for their digital scanner.  Again, millions versus
> thousands.
> >
> >
> >  Maybe we all need to hire publicity consultants
> or agents to get 
> >the word out... :)
> >
> >
> >
> >Claire Ingles
> >
> >UW Hospital
> >
> >Madison WI
> -- 
> Philip Oshel
> Microscopy Facility Supervisor
> Biology Department
> 024C Brooks Hall
> Central Michigan University
> Mt. Pleasant, MI 48859
> voice: (989) 774-3576
> dept. fax: (989) 774-3462
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 




------------------------------

Message: 13
Date: Wed, 11 Apr 2007 14:22:29 -0500
From: "Horn, Hazel V" 
Subject: RE: [Histonet] FW: HTL question
To: "Dolores Townsend" ,	"Jeanine
	(CDC/CCID/NCZVED) Bartlett" ,
	histonet@lists.utsouthwestern.edu
Message-ID:
	<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C5@EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii

I disagree.  An experienced pathologist will see and know fungus on an
H&E.   He/She does not need another stain, billed to the patient, for
confirmation.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: Wednesday, April 11, 2007 12:35 PM
To: Jeanine (CDC/CCID/NCZVED) Bartlett;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] FW: HTL question

As for a lot of tests, the answer is a bit tricky and confusing, but D
is right: although the pathologist can see something that MIGHT be
fungus on the routine stain, (s)he will ask for a special stain (GMS or
other) to confirm the presence of fungus. Hence, D. Fungi are never
demonstrated with routine procedure. You will need the special stain to
be sure. This is true for most special stains: the pathologist sees
something on the routine stain, but it is not conclusive, so special
stains are needed to confirm the diagnostic.
Dolores
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 14
Date: Wed, 11 Apr 2007 15:34:13 -0400
From: Philip Oshel 
Subject: RE: [Histonet] Re: Bone saw
To: Histonet@Pathology.swmed.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Mid-mitten Michigan. The way this weather system is moving, you may 
yet see snow. (I like Canada, but I could do without the exports from 
Nunavut and the Northwest Territories.)

And I only see the snow if I crawl up out of the dungeon where us EM 
trolls are generally kept.
Phil

>Hey Phil, where are you? I would love to see some snow.. rain here in
>Hotlanta.
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip
>Oshel
>Sent: Wednesday, April 11, 2007 1:48 PM
>To: Histonet@Pathology.swmed.edu
>Subject: RE: [Histonet] Re: Bone saw
>
>Rubber bracelets, H&E colored.
>Enjoy the snow today! 2 years ago, I would've been on the 10th floor
>of Animal Sciences, and I suspect unable to see the UW Hospital for
>the flakes.
>
>Phll
>
>>
>>
>>
>>
>>I know what you mean!  Here we can't get a tissue processor because the
>>Breast Radiology folks "forgot" to order all of the required components
>>for their digital scanner.  Again, millions versus thousands.
>>
>>
>>   Maybe we all need to hire publicity consultants or agents to get
>>the word out... :)
>>
>>
>>
>>Claire Ingles
>>
>>UW Hospital
>>
>>Madison WI
>--
>Philip Oshel
>Microscopy Facility Supervisor
>Biology Department
>024C Brooks Hall
>Central Michigan University
>Mt. Pleasant, MI 48859
>voice: (989) 774-3576
>dept. fax: (989) 774-3462
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>Confidentiality Notice ** The information contained in this message 
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------------------------------

Message: 15
Date: Wed, 11 Apr 2007 12:48:57 -0700 (PDT)
From: Akemi Allison-Tacha 
Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E
To: "Horn, Hazel V" ,	Dolores Townsend
	,	"Jeanine \(CDC/CCID/NCZVED\)
	Bartlett" ,	histonet@lists.utsouthwestern.edu
Message-ID: <862289.72421.qm@web31315.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

You have opened Pandora's Box!!
One thing I have learned, once it's in writing, no
turning back!

  --- "Horn, Hazel V"  wrote:

> I disagree.  An experienced pathologist will see and
> know fungus on an
> H&E.   He/She does not need another stain, billed to
> the patient, for
> confirmation.
> 
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall    Slot 820
> Little Rock, AR   72202
> 
> phone   501.364.4240
> fax        501.364.3912
> 
> visit us on the web at:    www.archildrens.org
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]
> On Behalf Of Dolores
> Townsend
> Sent: Wednesday, April 11, 2007 12:35 PM
> To: Jeanine (CDC/CCID/NCZVED) Bartlett;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] FW: HTL question
> 
> As for a lot of tests, the answer is a bit tricky
> and confusing, but D
> is right: although the pathologist can see something
> that MIGHT be
> fungus on the routine stain, (s)he will ask for a
> special stain (GMS or
> other) to confirm the presence of fungus. Hence, D.
> Fungi are never
> demonstrated with routine procedure. You will need
> the special stain to
> be sure. This is true for most special stains: the
> pathologist sees
> something on the routine stain, but it is not
> conclusive, so special
> stains are needed to confirm the diagnostic.
> Dolores
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
>
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> privileged and confidential
> and protected from disclosure. If the reader of this
> message is not the 
> intended recipient, or an employee or agent
> responsible for delivering this 
> message to the intended recipient, you are hereby
> notified that any 
> dissemination, distribution or copying of this
> communication is strictly 
> prohibited. If you have received this communication
> in error, please notify 
> us immediately by replying to the message and
> deleting it from your computer.
> Thank you.
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> 
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>
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------------------------------

Message: 16
Date: Wed, 11 Apr 2007 15:02:23 -0500
From: "Horn, Hazel V" 
Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E
To: "Akemi Allison-Tacha" ,	"Dolores Townsend"
	,	"Jeanine (CDC/CCID/NCZVED) Bartlett"
	,	histonet@lists.utsouthwestern.edu
Message-ID:
	<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C8@EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii

Oops....

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] 
Sent: Wednesday, April 11, 2007 2:49 PM
To: Horn, Hazel V; Dolores Townsend; Jeanine (CDC/CCID/NCZVED) Bartlett;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] FW: HTL question Can diagnose fungus from H&E

You have opened Pandora's Box!!
One thing I have learned, once it's in writing, no
turning back!

  --- "Horn, Hazel V"  wrote:

> I disagree.  An experienced pathologist will see and
> know fungus on an
> H&E.   He/She does not need another stain, billed to
> the patient, for
> confirmation.
> 
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall    Slot 820
> Little Rock, AR   72202
> 
> phone   501.364.4240
> fax        501.364.3912
> 
> visit us on the web at:    www.archildrens.org
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]
> On Behalf Of Dolores
> Townsend
> Sent: Wednesday, April 11, 2007 12:35 PM
> To: Jeanine (CDC/CCID/NCZVED) Bartlett;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] FW: HTL question
> 
> As for a lot of tests, the answer is a bit tricky
> and confusing, but D
> is right: although the pathologist can see something
> that MIGHT be
> fungus on the routine stain, (s)he will ask for a
> special stain (GMS or
> other) to confirm the presence of fungus. Hence, D.
> Fungi are never
> demonstrated with routine procedure. You will need
> the special stain to
> be sure. This is true for most special stains: the
> pathologist sees
> something on the routine stain, but it is not
> conclusive, so special
> stains are needed to confirm the diagnostic.
> Dolores
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
>
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------
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> privileged and confidential
> and protected from disclosure. If the reader of this
> message is not the 
> intended recipient, or an employee or agent
> responsible for delivering this 
> message to the intended recipient, you are hereby
> notified that any 
> dissemination, distribution or copying of this
> communication is strictly 
> prohibited. If you have received this communication
> in error, please notify 
> us immediately by replying to the message and
> deleting it from your computer.
> Thank you.
>
==========================3D=========================3D=====================
======
> 
> 
> _______________________________________________
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>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



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------------------------------

Message: 17
Date: Wed, 11 Apr 2007 14:04:49 -0600
From: Gayle Callis 
Subject: [Histonet] Seasonal snap freezing noses and ears in Montana 
To: Philip Oshel ,
	Histonet@lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20070411134619.01b0bf58@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Ah, you guys have it WARM, so don't be wussies!  It has been snowing here 
all week.  Come visit Montana where our annual Easter Bunny snowstorm only 
dropped 8 inches heavy wet white UGH! this year (normal is ~ 2 
ft!)  Temperatures plunged to 20F - 25F with winds making the annual Easter 
egg hunt a ear and nose snap freezing experience.  The front yard mini 
glacier should be taken out with Global Warming by end of week though.

Springtime in the Rockies is sooo much fun at times.

Gayle Callis
 From Frozen Reaches of Montana!



At 01:34 PM 4/11/2007, you wrote:
>Mid-mitten Michigan. The way this weather system is moving, you may yet 
>see snow. (I like Canada, but I could do without the exports from Nunavut 
>and the Northwest Territories.)
>
>And I only see the snow if I crawl up out of the dungeon where us EM 
>trolls are generally kept.
>Phil
>
>>Hey Phil, where are you? I would love to see some snow.. rain here in
>>Hotlanta.
>>
>>-----Original Message-----
>>From: histonet-bounces@lists.utsouthwestern.edu
>>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip
>>Oshel
>>Sent: Wednesday, April 11, 2007 1:48 PM
>>To: Histonet@Pathology.swmed.edu
>>Subject: RE: [Histonet] Re: Bone saw
>>
>>Rubber bracelets, H&E colored.
>>Enjoy the snow today! 2 years ago, I would've been on the 10th floor
>>of Animal Sciences, and I suspect unable to see the UW Hospital for
>>the flakes.
>>
>>Phll




------------------------------

Message: 18
Date: Wed, 11 Apr 2007 16:08:45 -0400
From: Philip Oshel 
Subject: [Histonet] web resource for histology
To: Histonet@Pathology.swmed.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Listers,

I just came upon this site and thought I'd pass it on. Hope it's not 
repeating a well-known resource.

http://courseweb.edteched.uottawa.ca/Medicine-histology/Default_En.htm

Also available in French.

Phil
-- 
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
voice: (989) 774-3576
dept. fax: (989) 774-3462



------------------------------

Message: 19
Date: Wed, 11 Apr 2007 15:12:54 -0500
From: "Rittman, Barry R" 
Subject: RE: [Histonet] Re: Bone saw
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"


While I don't wish to seem an old petarder, most of the several emails
we have seen re bone saws have nothing to do with bone saws. 
How about decreasing the amount of interpersonal chatter that is being
distributed to all on the Histonet so that we don't have to waste our
time with opening and deleting all these?
Thanks for your consideration
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: Wednesday, April 11, 2007 2:00 PM
To: Philip Oshel; Histonet@Pathology.swmed.edu
Subject: RE: [Histonet] Re: Bone saw

Yeah, Snow Brizzard in the middle of April...After
coming back from 75 degree weather.  I feel like like
Dorothy clicking her heels.... THERE'S NO PLACE LIKE
HOME!! THERE'S NO PLACE LIKE HOME!!  Won't be long
now.
Akemi
--- Philip Oshel  wrote:

> Rubber bracelets, H&E colored.
> Enjoy the snow today! 2 years ago, I would've been
> on the 10th floor 
> of Animal Sciences, and I suspect unable to see the
> UW Hospital for 
> the flakes.
> 
> Phll
> 
> >
> >
> >
> >
> >I know what you mean!  Here we can't get a tissue
> processor because the
> >Breast Radiology folks "forgot" to order all of the
> required components
> >for their digital scanner.  Again, millions versus
> thousands.
> >
> >
> >  Maybe we all need to hire publicity consultants
> or agents to get 
> >the word out... :)
> >
> >
> >
> >Claire Ingles
> >
> >UW Hospital
> >
> >Madison WI
> -- 
> Philip Oshel
> Microscopy Facility Supervisor
> Biology Department
> 024C Brooks Hall
> Central Michigan University
> Mt. Pleasant, MI 48859
> voice: (989) 774-3576
> dept. fax: (989) 774-3462
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


_______________________________________________
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Histonet@lists.utsouthwestern.edu
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------------------------------

Message: 20
Date: Wed, 11 Apr 2007 15:19:23 -0500
From: "Horn, Hazel V" 
Subject: RE: [Histonet] web resource for histology
To: "Philip Oshel" ,	Histonet@Pathology.swmed.edu
Message-ID:
	<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70C9@EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii

I browsed this site a bit and it is good.   I'll spend more time there
when I can.   

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip
Oshel
Sent: Wednesday, April 11, 2007 3:09 PM
To: Histonet@Pathology.swmed.edu
Subject: [Histonet] web resource for histology

Listers,

I just came upon this site and thought I'd pass it on. Hope it's not 
repeating a well-known resource.

http://courseweb.edteched.uottawa.ca/Medicine-histology/Default_En.htm

Also available in French.

Phil
-- 
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
voice: (989) 774-3576
dept. fax: (989) 774-3462

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------------------------------------------------------
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.
==========================3D=========================3D=========================3D==




------------------------------

Message: 21
Date: Wed, 11 Apr 2007 18:11:53 -0400
From: "Richard Cartun" 
Subject: Re: [Histonet] When staining IHC's, are labs putting control
	tissue on every slide?
To: ,	"James F. Happel"
	
Message-ID: <461D24E902000077000053E6@gwmail.harthosp.org>
Content-Type: text/plain; charset=US-ASCII

No, our positive control tissues are placed on separate slides.  We will cut 15-60 slides at a time depending on how often the control is used and the target protein's stability.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Happel, James F."  04/10/07 4:29 PM >>>
Good Afternoon All,

When staining IHC's, is anyone/everyone putting control tissue on every slide?
Both + and - ?

James

-----Original Message-----
From: Liz Beitman [mailto:LBeitman@cellmarque.com] 
Sent: Friday, March 23, 2007 12:19 PM
To: Happel, James F.
Subject: RE: [Histonet] Question about historical photographs

Hi James,

I just want to mention that we do have some posters of all the paintings
that adorn our catalog that I can send to you.  If you take a look in
our catalog (volume 7), page 79, it will give you an idea of some of the
posters.  Some are rather interesting from different points of view of
featured artists (Picasso, Klimt, and Michelangelo).  They might fit in
with your history presentation.  If you find any of interest, I would
look into either getting you an image or the actually poster.  Please
let me know.

Thanks,

Liz Beitman
Cell Marque
1-800-665-7284 x 8981
lbeitman@cellmarque.com 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Friday, March 23, 2007 7:49 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Question about historical photographs

Good Day,

I am putting together a presentation on the past, present and future of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital





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------------------------------

Message: 22
Date: Wed, 11 Apr 2007 18:20:25 -0400
From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" 
Subject: RE: [Histonet] FW: HTL question
To: "Horn, Hazel V" ,	"Dolores Townsend"
	,	
Message-ID:
	
Content-Type: text/plain;	charset="UTF-8"

Absolutely!   Sometimes you need that special for confirmation but definteley not always.

	-----Original Message----- 
	From: Horn, Hazel V [mailto:HornHV@archildrens.org] 
	Sent: Wed 4/11/2007 3:22 PM 
	To: Dolores Townsend; Bartlett, Jeanine (CDC/CCID/NCZVED); histonet@lists.utsouthwestern.edu 
	Cc: 
	Subject: RE: [Histonet] FW: HTL question
	
	

	I disagree.  An experienced pathologist will see and know fungus on an
	H&E.   He/She does not need another stain, billed to the patient, for
	confirmation.
	
	Hazel Horn
	Hazel Horn, HT/HTL (ASCP)
	Supervisor of Histology
	Arkansas Children's Hospital
	800 Marshall    Slot 820
	Little Rock, AR   72202
	
	phone   501.364.4240
	fax        501.364.3912
	
	visit us on the web at:    www.archildrens.org
	
	-----Original Message-----
	From: histonet-bounces@lists.utsouthwestern.edu
	[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
	Townsend
	Sent: Wednesday, April 11, 2007 12:35 PM
	To: Jeanine (CDC/CCID/NCZVED) Bartlett;
	histonet@lists.utsouthwestern.edu
	Subject: Re: [Histonet] FW: HTL question
	
	As for a lot of tests, the answer is a bit tricky and confusing, but D
	is right: although the pathologist can see something that MIGHT be
	fungus on the routine stain, (s)he will ask for a special stain (GMS or
	other) to confirm the presence of fungus. Hence, D. Fungi are never
	demonstrated with routine procedure. You will need the special stain to
	be sure. This is true for most special stains: the pathologist sees
	something on the routine stain, but it is not conclusive, so special
	stains are needed to confirm the diagnostic.
	Dolores
	_______________________________________________
	Histonet mailing list
	Histonet@lists.utsouthwestern.edu
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
	
	
	------------------------------------------------------------------------------
	The information contained in this message may be privileged and confidential
	and protected from disclosure. If the reader of this message is not the
	intended recipient, or an employee or agent responsible for delivering this
	message to the intended recipient, you are hereby notified that any
	dissemination, distribution or copying of this communication is strictly
	prohibited. If you have received this communication in error, please notify
	us immediately by replying to the message and deleting it from your computer.
	Thank you.
	=3D=========================3D=========================3D=========================3D==
	
	


------------------------------

Message: 23
Date: Wed, 11 Apr 2007 18:21:59 -0400
From: "Richard Cartun" 
Subject: Re: [Histonet] FW: HTL question
To: "Jeanine (CDC/CCID/NCZVED) Bartlett" ,
	
Message-ID: <461D274702000077000053EF@gwmail.harthosp.org>
Content-Type: text/plain; charset=US-ASCII

I would have picked "C" as well.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Bartlett, Jeanine (CDC/CCID/NCZVED)"  04/11/07 1:23 PM >>>
All,

I am forwarding this email from a young lady I know that is studying for
her HTL.  I will pass along your responses.  Thanks in advance for
helping.

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov 



> ______________________________________________ 
> From: 	Packard, Michelle (CDC/CCID/NCZVED)  
> Sent:	Wednesday, April 11, 2007 1:21 PM
> To:	Bartlett, Jeanine (CDC/CCID/NCZVED)
> Subject:	RE: HTL question
> 
> The following question is in the Histotechnology BOR Study Guide
> Practice Questions 2nd Edition published by ASCP:
> 
> For light microscopic evaluation it is generally necessary to use
> special stains to demonstrate fungi in tissue sections because fungi:
> A. can only be seen using silver impregnation
> B. are removed in the routine staining process
> C. stain variably with the H&E procedure
> D. are never demonstrated with routine procedures
> 
> The consensus here is that C is the best answer, however, the answer
> key lists D as correct.  Would like to get some feedback on this if
> possible.  Is the answer key wrong or am I missing something?  
> 
> In preparing for the exam, has anyone run across any other answers
> from this text that appear to be wrong?  
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