If possible and you can use resins, try Spurr's. It was specifically designed
to use with plants and vegetables, and you can cut as thin as 1 micron. The
resin is easy to use, is less toxic that epon, and has great penetration.
Heather Downs BS
Nerve Injury Unit
Mass General Hospital
Boston Mass, 02114
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Friday, April 13, 2007 11:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 41, Issue 20
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: Hello all (Akemi Allison-Tacha)
2. Re: Hello all (Akemi Allison-Tacha)
3. Re: Hello all (Akemi Allison-Tacha)
4. Re: Hello all (Akemi Allison-Tacha)
5. Sorry-Internet problems (Akemi Allison-Tacha)
6. Job Opening in East Texas (Susan Plaza)
7. ASCP exam stats July-Dec. 2006 (Lee & Peggy Wenk)
8. rodent smooth muscle marker (Jacqui Detmar)
9. RE: rodent smooth muscle marker (Liz Chlipala)
10. RE: rodent smooth muscle marker (Andrea Hooper)
11. plant anatomy - sectioning problems (yvan lindekens)
12. Demonstration of H. Pylori (Dolores Townsend)
13. RE: Demonstration of H. Pylori (Weems, Joyce)
14. RE: Demonstration of H. Pylori
(Marshall Terry Dr, Consultant Histopathologist)
15. Microwaves for use in antigen retrieval.
(Susan.Ferrigon@sanofi-aventis.com)
16. RE: Demonstration of H. Pylori (Douglas D Deltour)
17. Re: rodent smooth muscle marker (Geoff McAuliffe)
18. Re: Demonstration of H. Pylori (James L Burchette)
19. Re: plant anatomy - sectioning problems (Wurdak, Elizabeth)
20. RE: Demonstration of H. Pylori (Bonner, Janet)
21. H. Pylori IHC (Dolores Townsend)
22. Fluorescein Question (Breeden, Sara)
23. RE: Demonstration of H. Pylori (Rene J Buesa)
24. RE: H. Pylori IHC (Horn, Hazel V)
25. Re: H. Pylori IHC (James L Burchette)
26. CoverslippingRe: [Histonet] Fluorescein Question (Gayle Callis)
----------------------------------------------------------------------
Message: 1
Date: Thu, 12 Apr 2007 10:15:02 -0700 (PDT)
From: Akemi Allison-Tacha
Subject: Re: [Histonet] Hello all
To: Robyn Vazquez ,
Histonet@lists.utsouthwestern.edu
Message-ID: <420966.4499.qm@web31306.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
A couple of weeks ago there was quite a bit of
discussion on that subject. If you go to the histonet
archives and pull up H&E artifacts, it will give you
the book info with contacts.
Akemi Allison-Tacha
--- Robyn Vazquez wrote:
> Hello all
>
> Would anyone know where I could look for a
> troubleshooting guide for H
> & E staining?
>
> Thanks in advance
>
> Robyn
> OHSU
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 2
Date: Thu, 12 Apr 2007 10:15:10 -0700 (PDT)
From: Akemi Allison-Tacha
Subject: Re: [Histonet] Hello all
To: Robyn Vazquez ,
Histonet@lists.utsouthwestern.edu
Message-ID: <676225.38557.qm@web31301.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
A couple of weeks ago there was quite a bit of
discussion on that subject. If you go to the histonet
archives and pull up H&E artifacts, it will give you
the book info with contacts.
Akemi Allison-Tacha
--- Robyn Vazquez wrote:
> Hello all
>
> Would anyone know where I could look for a
> troubleshooting guide for H
> & E staining?
>
> Thanks in advance
>
> Robyn
> OHSU
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 3
Date: Thu, 12 Apr 2007 10:15:11 -0700 (PDT)
From: Akemi Allison-Tacha
Subject: Re: [Histonet] Hello all
To: Robyn Vazquez ,
Histonet@lists.utsouthwestern.edu
Message-ID: <457793.87073.qm@web31311.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
A couple of weeks ago there was quite a bit of
discussion on that subject. If you go to the histonet
archives and pull up H&E artifacts, it will give you
the book info with contacts.
Akemi Allison-Tacha
--- Robyn Vazquez wrote:
> Hello all
>
> Would anyone know where I could look for a
> troubleshooting guide for H
> & E staining?
>
> Thanks in advance
>
> Robyn
> OHSU
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 4
Date: Thu, 12 Apr 2007 10:15:12 -0700 (PDT)
From: Akemi Allison-Tacha
Subject: Re: [Histonet] Hello all
To: Robyn Vazquez ,
Histonet@lists.utsouthwestern.edu
Message-ID: <82803.73928.qm@web31302.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
A couple of weeks ago there was quite a bit of
discussion on that subject. If you go to the histonet
archives and pull up H&E artifacts, it will give you
the book info with contacts.
Akemi Allison-Tacha
--- Robyn Vazquez wrote:
> Hello all
>
> Would anyone know where I could look for a
> troubleshooting guide for H
> & E staining?
>
> Thanks in advance
>
> Robyn
> OHSU
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 5
Date: Thu, 12 Apr 2007 10:33:14 -0700 (PDT)
From: Akemi Allison-Tacha
Subject: [Histonet] Sorry-Internet problems
To: histonet@lists.utsouthwestern.edu
Message-ID: <897651.79340.qm@web31308.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Sorry for the multiple H&E responses. My connection
is messed-up.
Akemi
------------------------------
Message: 6
Date: Thu, 12 Apr 2007 12:38:23 -0500
From: "Susan Plaza"
Subject: [Histonet] Job Opening in East Texas
To: , "':'"
Message-ID: <000201c77d29$5ef145f0$800aa8c0@domain.local>
Content-Type: text/plain; charset="us-ascii"
Full-time Histotechnologist/Histologic Technician position available at
Pathology Associates of Tyler in scenic East Texas. Should be ASCP
registered or registry-eligible. P.A.T. offers a highly competitive
salary with an excellent benefits package. Interested applicants should
forward their resume to:
Pathology Associates of Tyler, 1726 South Beckham Ave., Tyler, TX
75701.
Fax: (903)592-0555. Phone: (903)593-0481 or e-mail:
sgibson@tylerpathology.com
------------------------------
Message: 7
Date: Thu, 12 Apr 2007 20:03:26 -0400
From: "Lee & Peggy Wenk"
Subject: [Histonet] ASCP exam stats July-Dec. 2006
To:
Message-ID: <000001c77d5f$2a738f50$a6bb2e4b@HPPav2>
Content-Type: text/plain; charset="us-ascii"
In case anyone wants the HT and HTL exam statistics for July-Dec 2006.
If you want information on other categories or other years, go to:
http://www.ascp.org/Certification/ForProgramDirectors/default.aspx
Couple of notes first:
- Range of scores is easiest question 100 to hardest question 999. Same with
practical exam score numbers.
- Minimul score of 400 degree of difficulty is required to pass each part.
- Both the practical (PRAC) and the multiple choice questions (MCQ) must be
passed, in order to pass the complete (COMP) exam. However, each candidates
has 5 tries, so they may pass one part one year, and try again the next year
- which is why the numbers don't match up.
- NAACLS is the National Accrediting Agency for Clinical Laboratory
Sciences. It is the agency that accredits HT and HTL programs. ASCP records
the number of NAACLS students taking the exam for the first time.
- The other category on the website is Total # Taking Exam. This would be
NAACLS students taking it for the first time, repeating NAACLS students, OJT
(on the job training) candidates taking it for the first time, and OJT
candidates repeating the exam.
- The "Non-NAACLS" category is one I extrapolated. It is the total # minus
the NAACLS first timers. That leave the OJT first timers, OJT repeaters, and
NAACLS repeaters (small number).
HT MCQ
- Mean = 432
- Range of Scores = 145-749
- Total # taking exam = 228
- Total pass = 134 = 59%
- NAACLS students taking exam = 86
- NAACLS students passing = 72 = 86%
- Non-NAACLS taking exam = 142
- Non-NAACLS passing = 62 = 44%
HT PRAC
- Mean = 543
- Range of Scores = 100-971
- Total # taking exam = 192
- Total pass = 166 = 57%
- NAACLS students taking exam = 117
- NAACLS students passing = 109 = 93%
- Non-NAACLS taking exam = 65
- Non-NAACLS passing = 57 = 88%
HT COMP
- Total # taking exam = 262
- Total pass = 189 = 72%
- NAACLS students taking exam = 64
- NAACLS students passing = 62 = 93%
- Non-NAACLS taking exam = 198
- Non-NAACLS passing = 127 = 64%
First year certified = 1948
Total Certified to date = 20,087
- - - - - -
HTL MCQ
- Mean = 434
- Range of Scores = 162-681
- Total # taking exam = 56
- Total pass = 39 = 70%
- NAACLS students taking exam = 7
- NAACLS students passing = 7 = 100%
- Non-NAACLS taking exam = 49
- Non-NAACLS passing = 32 = 65%
HTL PRAC
- Mean = 550
- Range of Scores = 325 - 736
- Total # taking exam = 43
- Total pass = 39 = 91%
- NAACLS students taking exam = 8
- NAACLS students passing = 8 = 100%
- Non-NAACLS taking exam = 35
- Non-NAACLS passing = 31 = 86%
HTL COMP
- Total # taking exam = 46
- Total pass = 40 = 87%
- NAACLS students taking exam = 8
- NAACLS students passing = 8 = 100%
- Non-NAACLS taking exam = 38
- Non-NAACLS passing = 32% = 84%
First year certified = 1980
Total Certified to date = 2,372
- - - - - -
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, mI 48073
------------------------------
Message: 8
Date: Thu, 12 Apr 2007 21:46:31 -0400
From: "Jacqui Detmar"
Subject: [Histonet] rodent smooth muscle marker
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hi all. I am asking this question on behalf of a colleague. She wishes to
measure rat uterine smooth muscle cell volume over gestation and would like to
have a marker that would delinate the smooth muscle plasma membrane. I have
looked at some of my Masson trichrome-stained mouse placental sections and this
*might* be helpful for her; however, I was thinking one of you might know of a
good lectin or antibody marker. Like I said, she works on rat tissue, but I
wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers,
too.
Thanks a bunch!
Jacqui Detmar
Samuel Lunenfeld Research Institute, room 876
Mount Sinai Hospital
600 University Avenue
Toronto, ON M5G 1X5
------------------------------
Message: 9
Date: Thu, 12 Apr 2007 20:12:44 -0600
From: "Liz Chlipala"
Subject: RE: [Histonet] rodent smooth muscle marker
To: "'Jacqui Detmar'" ,
Message-ID: <000001c77d71$39a3a1b0$0d00a8c0@domain.Premier>
Content-Type: text/plain; charset="us-ascii"
What about alpha smooth muscle actin, most anti-human SMA antibodies will
cross react with rodent. If she is planning on both rat and mouse tissues
then I would choose a rabbit antibody to smooth muscle actin. The one that
we use works in both rat and mouse, its from abcam ab5694.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui
Detmar
Sent: Thursday, April 12, 2007 7:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] rodent smooth muscle marker
Hi all. I am asking this question on behalf of a colleague. She wishes to
measure rat uterine smooth muscle cell volume over gestation and would like
to have a marker that would delinate the smooth muscle plasma membrane. I
have looked at some of my Masson trichrome-stained mouse placental sections
and this *might* be helpful for her; however, I was thinking one of you
might know of a good lectin or antibody marker. Like I said, she works on
rat tissue, but I wouldn't mind receiving some advice on mouse uterine
smooth muscle cell markers, too.
Thanks a bunch!
Jacqui Detmar
Samuel Lunenfeld Research Institute, room 876
Mount Sinai Hospital
600 University Avenue
Toronto, ON M5G 1X5
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
__________ NOD32 1971 (20070110) Information __________
This message was checked by NOD32 antivirus system.
http://www.eset.com
------------------------------
Message: 10
Date: Thu, 12 Apr 2007 22:31:45 -0400
From: "Andrea Hooper"
Subject: RE: [Histonet] rodent smooth muscle marker
To: Liz Chlipala , Histonet
Message-ID:
Content-Type: text/plain; charset=us-ascii; format=flowed
Along those same lines mouse anti-SMAalpha clone 1A4 works very well
for SMC in mouse and human. To avoid having to use a MOM kit I use
the EPOS version from DAKO which is VERY pricey but worth every penny
for the gorgeous and clean result in mouse tissues (it can also be
diluted and doesn't need to be used neat as recommended by
manufacturer).
Andrea
>What about alpha smooth muscle actin, most anti-human SMA antibodies will
>cross react with rodent. If she is planning on both rat and mouse tissues
>then I would choose a rabbit antibody to smooth muscle actin. The one that
>we use works in both rat and mouse, its from abcam ab5694.
>
>Liz
>
>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>Manager
>Premier Laboratory, LLC
>P.O. Box 18592
>Boulder, CO 80308
>phone (303) 735-5001
>fax (303) 735-3540
>liz@premierlab.com
>www.premierlab.com
>
>Ship to Address:
>
>Premier Laboratory, LLC
>University of Colorado at Boulder
>MCDB, Room A3B40
>Boulder, CO 80309
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui
>Detmar
>Sent: Thursday, April 12, 2007 7:47 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] rodent smooth muscle marker
>
>Hi all. I am asking this question on behalf of a colleague. She wishes to
>measure rat uterine smooth muscle cell volume over gestation and would like
>to have a marker that would delinate the smooth muscle plasma membrane. I
>have looked at some of my Masson trichrome-stained mouse placental sections
>and this *might* be helpful for her; however, I was thinking one of you
>might know of a good lectin or antibody marker. Like I said, she works on
>rat tissue, but I wouldn't mind receiving some advice on mouse uterine
>smooth muscle cell markers, too.
>
>Thanks a bunch!
>
>Jacqui Detmar
>Samuel Lunenfeld Research Institute, room 876
>Mount Sinai Hospital
>600 University Avenue
>Toronto, ON M5G 1X5
>
--
------------------------------
Message: 11
Date: Fri, 13 Apr 2007 02:17:47 -0700 (PDT)
From: yvan lindekens
Subject: [Histonet] plant anatomy - sectioning problems
To: histonet@lists.utsouthwestern.edu
Message-ID: <292946.36182.qm@web30909.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi all,
I'm trying to cut some plant samples but I have lots
of problems with the samples containing even the
smallest amount of wood (f.e. young white deadnettle
stems - Lamium album L.).
A thickness of 15 micron is the thinnest I can get,
without the ribbon scattering longitudaly after only a
few sections. Whiping the blade with a cloth moisted
with some xylene solves the problem, but only for
another 2 or 3 sections... I use a Leica-Jung Autocut
1140 and Feather S/R/N 35 blades.
Cutting at veeeeeeryyyyy looooow speed gives slightly
better results but still not good enough!
When sectioned on a sliding microtome(Reichert-Jung
Hn-40, Feather S/R/N 35 blades, declination angle +/-
130°), the sections are okay, but I would like to use
a rotary.
Samples were fixed in AFA, processed by hand using an
ETOH/IPA/xylene protocol. On the other hand I
processed some samples using a tert. butyl alcohol
schedule. There's hardly any difference between the
two regarding sectioning properties...
By the way: is there something as a "standard
thickness" for plantanatomical sections?
Thanks in advance for every suggestion!
Yvan.
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
------------------------------
Message: 12
Date: Fri, 13 Apr 2007 09:45:55 -0400
From: "Dolores Townsend"
Subject: [Histonet] Demonstration of H. Pylori
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
------------------------------
Message: 13
Date: Fri, 13 Apr 2007 09:49:50 -0400
From: "Weems, Joyce"
Subject: RE: [Histonet] Demonstration of H. Pylori
To: "Dolores Townsend" ,
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD32037D3BD1@sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
We do Genta - actually a modified Genta. We have the Artisan - so we do a
Warthin Starry and add the rest off line. No IHC.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dolores
Townsend
Sent: Friday, April 13, 2007 9:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Demonstration of H. Pylori
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice ** The information contained in this message may be
privileged and is confidential information intended for the use of the addressee
listed above. If you are neither the intended recipient nor the employee or
agent responsible for delivering this message to the intended recipient, you are
hereby notified that any disclosure, copying, distribution or the taking of any
action in reliance on the contents of this information is strictly prohibited.
If you have received this communication in error, please notify us immediately
by replying to the message and deleting it from your computer. Thank you. Saint
Joseph's Health System, Inc.
------------------------------
Message: 14
Date: Fri, 13 Apr 2007 15:00:12 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
Subject: RE: [Histonet] Demonstration of H. Pylori
To: "Dolores Townsend" ,
Message-ID:
<407F05A128805F4C879A33DBA32E618E20C2BD@TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
I am replying on behalf of my technologists to say that their
pathologist likes ipox, though usually does with an H & E.
He asks for it when there is an appearance which suggests H. pylori
should be there but can't see it, or when he sees organisms but they are
not typical shape. (H. pylori occasionally has a coccal form).
One of the girls did a project comparing 6 or 7 stains and the best was
ipox,as one would expect. The next best was Giema, in that it gave the
best staining against background light staining, most consistently.
Silver stains were pretty hopeless. Tol. Blue easily overstained, and
others were too laborious.
Terry
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: 13 April 2007 14:46
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Demonstration of H. Pylori
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave, and
seems to give variable results in staining intensity, so I'd like your
opinion as to what stain you prefer.
Thank you,
Dolores Townsend
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Fri, 13 Apr 2007 15:02:31 +0100
From:
Subject: [Histonet] Microwaves for use in antigen retrieval.
To:
Message-ID:
<90B6684A9D6DAF468F7A5DC148754E1D1E197B@ALPW31.f2.enterprise>
Content-Type: text/plain; charset="us-ascii"
Hi
I would like to purchase a microwave to use in our ICC lab for antigen
retrieval, can anyone recommend a reliable laboratory microwave???( at
present we have a old household Sanyo model but would like to buy a new
one where we can accurately measure the internal temperature).
Thanks
Susan
------------------------------
Message: 16
Date: Fri, 13 Apr 2007 10:08:22 -0500
From: "Douglas D Deltour"
Subject: RE: [Histonet] Demonstration of H. Pylori
To: "'Dolores Townsend'" ,
Message-ID:
Content-Type: text/plain; charset="us-ascii"
We do IHC.
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: Friday, April 13, 2007 8:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Demonstration of H. Pylori
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Fri, 13 Apr 2007 10:14:51 -0400
From: Geoff McAuliffe
Subject: Re: [Histonet] rodent smooth muscle marker
To: Jacqui Detmar
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <461F905B.3030509@umdnj.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
Periodic acid-Shiff (PAS) will outline the smooth muscle cells by
staining the cell coat external to the plasma membrane.
Geoff
Jacqui Detmar wrote:
>Hi all. I am asking this question on behalf of a colleague. She wishes to
measure rat uterine smooth muscle cell volume over gestation and would like to
have a marker that would delinate the smooth muscle plasma membrane. I have
looked at some of my Masson trichrome-stained mouse placental sections and this
*might* be helpful for her; however, I was thinking one of you might know of a
good lectin or antibody marker. Like I said, she works on rat tissue, but I
wouldn't mind receiving some advice on mouse uterine smooth muscle cell markers,
too.
>
>Thanks a bunch!
>
>Jacqui Detmar
>Samuel Lunenfeld Research Institute, room 876
>Mount Sinai Hospital
>600 University Avenue
>Toronto, ON M5G 1X5
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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------------------------------
Message: 18
Date: Fri, 13 Apr 2007 09:21:14 -0500
From: James L Burchette
Subject: Re: [Histonet] Demonstration of H. Pylori
To: "Dolores Townsend"
Cc: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Hello Delores and everyone else,
We use IHC for the demonstration of H pylori. I don't get caught up in the=20
argument of IHC vs. special stains. If our doctors like IHC, that's fine
with me. Job security says it all.
I use Dako's rabbit polyclonal antibody at 1:100, pretreat with Brigati's=20
stable pepsin, and detect with a biotin/streptavidin system. I like AEC,
but sometimes use DAB as the chromogen.
After taking a couple years off from Histonet.... I'm back
Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"
"Dolores Townsend"
Sent by: histonet-bounces@lists.utsouthwestern.edu
04/13/2007 08:45 AM
To
histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] Demonstration of H. Pylori
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Fri, 13 Apr 2007 09:29:21 -0500
From: "Wurdak, Elizabeth"
Subject: Re: [Histonet] plant anatomy - sectioning problems
To: yvan lindekens , Histonet
Message-ID:
Content-Type: text/plain; charset="ISO-8859-1"
Hi Yvan,
The procedure we followed is listed below. We had pretty good results as
far as sectioning went. We are staining with safranin and fast green or
hematoxylin and safranin. Neither comes out as bright as I would like to
see it. I would welcome any suggestions you have for staining.
Liz
Processing Plant Tissues for Paraffin Sections
On January 5 and 6, 2007 various plant organs were collected in the SJU
Greenhouse. They were fixed in FAA and kept in this solution until February
13 and 14, 2007.
The composition of FAA is as follows:
95% ethanol 50 cc
glacial acetic acid 5 cc
formalin 10 cc
distilled water 35 cc
Wiley, R. L. 1971 Microtechniques: A Laboratory Guide, The Macmillan
Company, New York
Dehydration and clearing was carried out according to the following
schedule:
1. Jar #1: 30:50:20 water:alcohol:tert-butyl-alcohol
2. Jar #2: 15:50:35 water:alcohol:tert-butyl-alcohol for 1 hour.
3. Jar #3: 25:75 alcohol:tert-butyl-alcohol for 1 hour.
4. Jar #4: 100% Tert-butyl-alcohol for 1 hour.
5. Jar #5: 100% Tert-butyl-alcohol for 1 hour.
6. Jar #6: 100% Tert-butyl-alcohol + paraffin chips, ON at 45oC.
7. Jar #7: Pure paraffin 30 min, 60oC under vacuum
8. Jar #8: Pure paraffin in 60oC oven 1hr.
9. Jar #9: Third change of pure paraffin in 60oC oven 1hr.
10. Embed.
This schedule is a modified version of the protocol we followed in 1997. I
also consulted
Grey, P. 1964 Handbook of Basic Microtechnique 3rd. ed, McGraw-Hill Book
Company, New York.
Ruzin, S. E. 1999 Plant Microtechnique and Microscopy, Oxford University
Press, New York
On 4/13/07 4:17 AM, "yvan lindekens" wrote:
> Hi all,
>
> I'm trying to cut some plant samples but I have lots
> of problems with the samples containing even the
> smallest amount of wood (f.e. young white deadnettle
> stems - Lamium album L.).
>
> A thickness of 15 micron is the thinnest I can get,
> without the ribbon scattering longitudaly after only a
> few sections. Whiping the blade with a cloth moisted
> with some xylene solves the problem, but only for
> another 2 or 3 sections... I use a Leica-Jung Autocut
> 1140 and Feather S/R/N 35 blades.
>
> Cutting at veeeeeeryyyyy looooow speed gives slightly
> better results but still not good enough!
>
> When sectioned on a sliding microtome(Reichert-Jung
> Hn-40, Feather S/R/N 35 blades, declination angle +/-
> 130°), the sections are okay, but I would like to use
> a rotary.
>
> Samples were fixed in AFA, processed by hand using an
> ETOH/IPA/xylene protocol. On the other hand I
> processed some samples using a tert. butyl alcohol
> schedule. There's hardly any difference between the
> two regarding sectioning properties...
>
> By the way: is there something as a "standard
> thickness" for plantanatomical sections?
>
> Thanks in advance for every suggestion!
>
> Yvan.
>
> __________________________________________________
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> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Fri, 13 Apr 2007 10:37:21 -0400
From: "Bonner, Janet"
Subject: RE: [Histonet] Demonstration of H. Pylori
To: "Dolores Townsend" ,
histonet@lists.utsouthwestern.edu
Message-ID:
<5F31F38C96781A4FBE3196EBC22D47802E0F43@fhosxchmb006.ADVENTISTCORP.NET>
Content-Type: text/plain; charset=iso-8859-1
We do a Giemsa stain.
________________________________
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dolores Townsend
Sent: Fri 4/13/2007 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Demonstration of H. Pylori
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
==========================3D=========================3D====
The information contained in this message may be privileged and/or confidential
and protected from disclosure. If the reader of this message is not the
intended
recipient or an employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any dissemination, distribution
or copying of this communication is strictly prohibited. If you have received
this
communication in error, please notify the sender immediately by replying to this
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==========================3D=========================3D====
------------------------------
Message: 21
Date: Fri, 13 Apr 2007 10:46:37 -0400
From: "Dolores Townsend"
Subject: [Histonet] H. Pylori IHC
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
One more question for those who do IHC: do you do these routinely on all
gastric biopsies, or only as requested by your pathologist?
Thanks,
Dolores
------------------------------
Message: 22
Date: Fri, 13 Apr 2007 08:48:01 -0600
From: "Breeden, Sara"
Subject: [Histonet] Fluorescein Question
To:
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B8F43FD@nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
A current project is cutting slides to check for fluorescein expression.
The first time I did this, I was asked to air-dry the slides and mount
with aqueous medium; as you can expect, the coverage was "iffy" at best
with lifting of the coverslip, etc. Is there any reason I could not dry
these in a 60 degree oven as usual, then deparaffinize and coverslip out
of xylene? Would this process compromise the results? Help? Thanks!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 23
Date: Fri, 13 Apr 2007 07:50:05 -0700 (PDT)
From: Rene J Buesa
Subject: RE: [Histonet] Demonstration of H. Pylori
To: "Bonner, Janet" , Dolores Townsend
,
histonet@lists.utsouthwestern.edu
Message-ID: <874731.20789.qm@web61224.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We do modified Steiner
René J.
"Bonner, Janet" wrote:
We do a Giemsa stain.
________________________________
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dolores Townsend
Sent: Fri 4/13/2007 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Demonstration of H. Pylori
Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave,
and seems to give variable results in staining intensity, so I'd like
your opinion as to what stain you prefer.
Thank you,
Dolores Townsend
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
==========================3D=========================3D====
The information contained in this message may be privileged and/or confidential
and protected from disclosure. If the reader of this message is not the intended
recipient or an employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any dissemination, distribution
or copying of this communication is strictly prohibited. If you have received
this
communication in error, please notify the sender immediately by replying to this
message and deleting the material from any computer.
==========================3D=========================3D====
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Ahhh...imagining that irresistible "new car" smell?
Check outnew cars at Yahoo! Autos.
------------------------------
Message: 24
Date: Fri, 13 Apr 2007 09:51:04 -0500
From: "Horn, Hazel V"
Subject: RE: [Histonet] H. Pylori IHC
To: "Dolores Townsend" ,
histonet@lists.utsouthwestern.edu
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C630ACB70CF@EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii
We do IHC only if requested by the pathologist.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: Friday, April 13, 2007 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H. Pylori IHC
One more question for those who do IHC: do you do these routinely on all
gastric biopsies, or only as requested by your pathologist?
Thanks,
Dolores
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------------------------------------------------------
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this=20
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify=20
us immediately by replying to the message and deleting it from your computer.
Thank you.
==========================3D=========================3D=========================3D==
------------------------------
Message: 25
Date: Fri, 13 Apr 2007 09:58:09 -0500
From: James L Burchette
Subject: Re: [Histonet] H. Pylori IHC
To: "Dolores Townsend"
Cc: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
It depends on the clinical history. Some GI bx's are accessioned and H
pylori is prospectively ordered. Other IHC requests come in as a direct
order from the attending pathologist or resident in training.
Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"
"Dolores Townsend"
Sent by: histonet-bounces@lists.utsouthwestern.edu
04/13/2007 09:46 AM
To
histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] H. Pylori IHC
One more question for those who do IHC: do you do these routinely on all
gastric biopsies, or only as requested by your pathologist?
Thanks,
Dolores
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 26
Date: Fri, 13 Apr 2007 09:16:24 -0600
From: Gayle Callis
Subject: CoverslippingRe: [Histonet] Fluorescein Question
To: "Breeden, Sara" ,
Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20070413090850.01b5f278@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Sara,
Try it, but keep sections in the dark to avoid photobleaching. Fluorescein=20
should survive deparaffinization and then coverslipping. Putting in an
extra xylene to ensure good paraffin removal before putting a coverglass on=20
may be of help. We have some who do that here but they are requesting
thick sections ~ 10 um or so. I generally just air dry at RT in a dark
place. If you want to know the effects of temperature on FITC, contact
Molecular Probes technical services, they will know about this also.
However, if the tissues are fixed with formalin, you may be battling
autofluorescence. At 08:48 AM 4/13/2007, you wrote:
>A current project is cutting slides to check for fluorescein expression.
>The first time I did this, I was asked to air-dry the slides and mount
>with aqueous medium; as you can expect, the coverage was "iffy" at best
>with lifting of the coverslip, etc. Is there any reason I could not dry
>these in a 60 degree oven as usual, then deparaffinize and coverslip out
>of xylene? Would this process compromise the results? Help? Thanks!
>
>
>
>Sally Breeden, HT(ASCP)
>
>NM Dept. of Agriculture
>
>Veterinary Diagnostic Services
>
>PO Box 700
>
>Albuquerque, NM 87106
>
>505-841-2576
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
------------------------------
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