Re: [Histonet] repeating AR heat treatment

From:"Joe Nocito"

my experience is that I didn't need to go through the retrieval steps again. 
I've done more cases than I can count with a pathologist wanting one immuno, 
reads that one and then orders another one on the same slide. Just my 3 
cents worth.

Histology Manager
Pathology Reference Lab
San Antonio, TX

----- Original Message ----- 
From: "Bobrowitz, Carol" 
To: "'Histonet (E-mail)" 
Sent: Friday, April 21, 2006 11:29 AM
Subject: [Histonet] repeating AR heat treatment

I have already stained FFPE rat kidney's using FITC with a 2 hour heat 
treatment in citrate buffer ph 6.0.  (the staining was great)

I am now going to restain the same rat kidney slides with an additional 
primary using DAB as the chromagen.  This primary also requires heat 

Am I correct in my thinking that I should repeat the heat treatment again 
due to the fact that the epitope (binding) sites have already closed after 
accepting the application of the 1st primary?

Any help will be appreciated.  Thank you in advance.

Carol Ann Bobrowitz
Medical College of Wisconsin
Department of Physiology
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