I do use p27, but not your clone.  I use clone SX53G8  from Dako.  
They are a great company to deal with; very user friendly.
This p27 is IVD  cat.3 M7203;  I use it at 1:80 with their Target Retrieval Solution
and incubate for 15 min.  room temp.

Dana Settembre
University Hospital - UMDNJ
Newark, NJ
USA 

>>> Montse verdu  04/26/06 6:11 AM >>>
Dear histonetters,
 
Anybody has experience with p27 clone DSC72 different than Calbiochem
antibody? We use these antibody on formalin-fixed, paraffin-embedded
human prostate carcinoma stained, using avidin-biotin peroxidase complex
and DAB chromogen, and we are satisfied with the results, but we have
problems with the distributor of these product (frequently we received
products with an expiration date from before at the delivery date) and
for these reason we needs to change of antibody.
Thanks for your help.
 
Montse Verdú
histopat LABORATORIS
Tel. 932033000
Fax 932802160
mverdu@histopat.es 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet