Re: [Histonet] Large vessel endothelium problem
Hello again. Sorry for a bit unclearly formatted question.
More specifically, I'm doing samples of bovine blood vessels, obtained
from a slaughterhouse. So perfusion is unfortunately out of question :)
And this is about REALLY big vessels, such as the bovine aorta. So the
samples are bits (about 1 cm2) of vessel wall cut off and placed in PFA
for overnight at +4C, then processed for paraffin sections in a routine
And yes, this is primarily about blood vessels, not lymphatics.
Gayle Callis wrote:
> You did not indicate how you do the paraformaldehyde fixation?
> Immersion or perfusion?
> Vascular perfusion followed by immersion for a few hours may help your
> problem - to ensure the tissue is fixed from the inside out and in
> particular the lumens of your large blood (?) vessels.
> You did not give details on what you do with the vessels (opened, etc,
> stretched out) during fixation or processing?
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mitäkö mieltä olen länsimaisesta sivistyksestä?
Minusta se olisi erinomainen ajatus!
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