Re: [Histonet] Large vessel endothelium problem

From:Gayle Callis

You did not indicate how you do the paraformaldehyde fixation?  Immersion 
or perfusion?

Vascular perfusion followed by immersion for a few hours may help your 
problem - to ensure the tissue is fixed from the inside out and in 
particular the lumens of your large blood (?) vessels.

You did not give details on what you do with the vessels (opened, etc, 
stretched out) during fixation or processing?

At 06:21 AM 4/18/2006, you wrote:
>I need to do some immunostaining of large vessel endothelium 
>(paraformaldehyde-fixed paraffin material).
>The problem is, the endothelium is easily stripped off and lost, 
>apparently in early steps of sample processing.
>Any tips how to keep the stuff intact?
>With best regards,
>  Mikael Niku             URL:
>  University of Helsinki  Dept. Basic Veterinary Sciences
>  - Mitäkö mieltä olen länsimaisesta sivistyksestä?
>  Minusta se olisi erinomainen 
> ajatus!
>                                          - Gandhi
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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