Re: [Histonet] Bone decalcification


do you not have any text books? 

 As you have already seen the majority of answers are saying fixation is 
of prime importance if you wish to observe the cellular components of the 
bone using microscopy.  Reason you want to decalcify is presumably to be 
able to cut nice thin paraffin wax sections?  No point in doing that if 
you've already ruined the morphology by not fixing appropriately.  Don't 
know how anyone could 'learn this the hard way'  do people really walk in 
to labs and make it up as they go along?

Gill Brown 
Sent by:
24-Apr-2006 11:40

[Histonet] Bone decalcification

  I just wanted to know other histo techs opinions on bone 
I learned in school that you decal the bone than you fix it. I have a
pathologist who claims she learned it the hard way, and that it is better 
fix the bone than decal it. What did you learn on how to do this 
This pathologist claims that fixing prior to decaling keeps the cells more
intact. Any opinion on your procedure or your technique is appreciated.


Heather A. Harper 

Naval Hospital 

Pensacola, FL

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