RE: [Histonet] special stain with a immunohistochemical stain

From:"Elizabeth Chlipala"


DAB as a chromagen is very stable, we run the immuno first and then the
special stain.  We have done this with DAB and then Acid fast, there is
also a nice Histologic article on this type of dual staining, I think it
was on T cells and PAS in kidney samples.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
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-----Original Message-----
[] On Behalf Of Dana
Sent: Monday, April 17, 2006 12:43 PM
To:; cynthia haynes
Subject: Re: [Histonet] special stain with a immunohistochemical stain

Good question, Cynthia.
I have only run immunos on slides that have had special stains run on
them already. They work. Dana Settembre University Hospital-UMDNJ
Newark, NJ

>>> cynthia haynes  04/17/06 2:02 PM >>>
Hello fellow Histonetters

A researcher gave me a DAB stained slides. He would
like for me to take the cover glass off and stain the
slide with LUXOL FAST BLUE. My question is when you
want to combine a special stain with
immunohistochemical stains, which should you do first?
The special stain or the immuno stain? Thanks in

Cynthia Haynes H.T.

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