Cynthia

DAB as a chromagen is very stable, we run the immuno first and then the
special stain.  We have done this with DAB and then Acid fast, there is
also a nice Histologic article on this type of dual staining, I think it
was on T cells and PAS in kidney samples.

Liz

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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Monday, April 17, 2006 12:43 PM
To: histonet@pathology.swmed.edu; cynthia haynes
Subject: Re: [Histonet] special stain with a immunohistochemical stain


Good question, Cynthia.
I have only run immunos on slides that have had special stains run on
them already. They work. Dana Settembre University Hospital-UMDNJ
Newark, NJ

>>> cynthia haynes  04/17/06 2:02 PM >>>
Hello fellow Histonetters

A researcher gave me a DAB stained slides. He would
like for me to take the cover glass off and stain the
slide with LUXOL FAST BLUE. My question is when you
want to combine a special stain with
immunohistochemical stains, which should you do first?
The special stain or the immuno stain? Thanks in
advance.

Cynthia Haynes H.T.

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