The immuno stain should ordinarily be done first, as it is practically
impossible to remove the DAB staining histochemically, while it is very
possible to remove or otherwise alter some of the histochemical dyes while
doing the immuno stain.  Just last week I did some trichromes on
previously-stained immuno slides.  The result was very nice.  They had
stained a marker in mouse heart, and then wanted to know if it was
associated with areas of fibrosis.  Wherever areas of DAB staining occurred,
they were surrounded by the blue-stained collagen fibers.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet