RE: [Histonet] Expiry Period - Immunofluorescence Dilutions

From:"Favara, Cynthia \(NIH/NIAID\) [E]"

You might want to check for yourself.

Make up the amount you would like to use and run a control slide weekly
until signal fades. I would make sure all other lot numbers are the
same. I would think that time exposed to light may be a significant
factor. Just a thought.

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: Gagnon, Eric [mailto:gagnone@KGH.KARI.NET] 
Sent: Tuesday, April 18, 2006 10:23 AM
Subject: [Histonet] Expiry Period - Immunofluorescence Dilutions

Hello Listers,
We currently do immunofluorescence for IgG, IgA, IgM, C3, C1q, Fib.,
Kappa, Lambda, and C4d on renal biopsies, and the first four on skin
biopsies.  We are using Dako Antibody Diluting Buffer and mostly Dako
FITC antisera.
We currently make up about 1 ml of each, and the dilutions last us
between 1 and 3 weeks.  We would like to keep them longer if possible,
making them up in larger quantities, possibly with the goal of
transferring them to dispensers on our Ventana XT's.
Is there anyone doing the same immunofluorescence panel and dilutions,
and if so, how long are you able to use your dilutions once made?
Thanks for your assistance,
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada

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