A Hair dryer helps with the drying.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, 20 April 2006 11:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Diff Quick


We run a one-step FNAC breast clinic and have been using Diff-Quick as
our stain; I personally don't like it as I prefer Paps but there you
are. We had a recent FNAC that showed histiocytes in the tail of the
prep but little else. As you know the bubbly cells tend to migrate to
the edges as they are lighter but what we initially failed to notice was
that in amongst the blood were poorly stained clumps of cells. After
restain with a 'proper' Romanovsky stain they magically appeared and
were benign. I shudder to think what would have happened if they had not
been spotted and had been malignant. The problem I guess was the blood
and together with the slow drying of the smear.

Diff-Quick is fast but can have this effect, does anyone have a
technique that is nearly as fast as Diff Quick but more robust in these
cases?

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

 

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Lao Tzu 

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