RE: [Histonet] Bone decalcification

From:"Sheffield, Tiffany L"

One of the most important things I learned back in the day was the
importance of choosing the appropriate fixative. In your case I would
always fix the tissue/bone first before decal.
Hope this helps :-) 


Tiffany Sheffield-Lopez,BS,HT(ASCP)

Supervisor, Bone Histomorphometry & Biomaterials Lab

Department of Orthopaedic Surgery

The University of Texas Houston Health Science Center

6431 Fannin, Suite 6.144 MSB

Houston, TX 77030

713-500-6803 Wk  713-500-0729 Fax

-----Original Message-----
[] On Behalf Of
Sent: Monday, April 24, 2006 5:40 AM
Subject: [Histonet] Bone decalcification

  I just wanted to know other histo techs opinions on bone
I learned in school that you decal the bone than you fix it. I have a
pathologist who claims she learned it the hard way, and that it is
better to fix the bone than decal it. What did you learn on how to do
this procedure?
This pathologist claims that fixing prior to decaling keeps the cells
more intact. Any opinion on your procedure or your technique is


Heather A. Harper          

Naval Hospital 

Pensacola, FL

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