[Histonet] secondary antibody

From:"louise renton"

Dear immuno experts,

Please help my soggy xylene soaked brain work out what is probably a
simple question. If I am using a polyclonal antibody on mouse tissue
(reactivity is established) , and detecting with Vector's ABC kit,
what biotinylated seconday do I use? goat or swine anti rabbit?
something else??? ?
PS I have a mouse on mouse kit already -are there regaents in there
that i could sucessfully use??
PPS. This is the first time i have done immuno in other species, I was
used to work on human tissue alone hence my confusion

Thanks in advance

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"Does a conceited cowboy, only ride on pompous grass?.

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