Co-localization of proteins is no a simple as a color change. You can get a color change in an section if the proteins are above and below each other in the section and still not occupying the same space. There is a very complicated formula(s) that can be used when calculating cooficients of co-localization and it is much more complicated than a color change. You should be using image analysis software and doing calculations of these co-efficients to get a true account of what is really happening. Check out http://www.mediacy.com/index.aspx?page=FT_Colocalization


Caron Fournier, BSc, R.T.
Department of Orthopaedics,
Division of Orthopaedic Engineering Research,
U.B.C.
 

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