[Histonet] TUNEL staining
|From:||"Kalleberg, Kristopher" |
I am currently performing TUNEL on human skin post UV irradiation and
have had inconclusive results. I was using the Promega TUNEL assay kit
and also tried a formamide induced TUNEL assay with differing results.
I also have read in the literature that the kit and most TUNEL assays
are nonspecific (necrotic cells also show positive staining). The
formamide induced protocol that I have only tried twice has shown no
positive staining yet. I was just wondering which protocol you use for
TUNEL and how certain are you that it is specific for apoptotic cells?
Maybe we can help each other.
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