[Histonet] RE: Ki67 protocol--frozen sections
For staining of Ki67 we like to fix human cryostat tissues
sections with Zamboni's or buffered formalin. This results into a
sharper nuclear staining than acetone-fixation. Just any polymer/HRP
visualization and DAB+ as chromogen will do.
As for a Ki67 antibody working on feline tissues I would recommend you
to test the SP6 clone (rabbit monoclonal) from LabVision or Vector. In
our hands this clone already worked for a lot of different species.
Perhaps you can expand the list with feline...
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Thu, 20 Apr 2006 22:21:29 -0400
From: "Janelle Novak"
Subject: [Histonet] Ki67 protocol--frozen sections
Does anyone have any good recommendations for a Ki67 staining protocol
for feline frozen tissue sections?
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