[Histonet] Over lapped flouresence in double staining
I am doing double staining of endothelial cells and smooth muscles of the blood vessels with vWF and SMA.
While doing double staining I have encountered overlapping of both antibodies and I cant see a clear differentiation between the fluorescence of these two antibodies.
Could you please guide me how to over come this problem.
Thanks
Dr. Sohail
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