[Histonet] Over lapped flouresence in double staining

From:sohail ejaz

I am doing double staining of endothelial cells and smooth muscles of the blood vessels with vWF and SMA.
   
  While doing double staining I have encountered overlapping of  both antibodies and I cant see a clear differentiation between the fluorescence of these two antibodies.
   
  Could you please guide me how to over come this problem.
   
  Thanks
   
  Dr. Sohail 
  

 

		
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