[Histonet] Large vessel endothelium problem

From:Mikael Niku


I need to do some immunostaining of large vessel endothelium 
(paraformaldehyde-fixed paraffin material).
The problem is, the endothelium is easily stripped off and lost, 
apparently in early steps of sample processing.
Any tips how to keep the stuff intact?

With best regards,


  Mikael Niku             URL: www.helsinki.fi/~mniku/
  University of Helsinki  Dept. Basic Veterinary Sciences

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