[Histonet] Expiry Period - Immunofluorescence Dilutions (Gagnon, Eric)
Have you considered asking your Ventana rep for information on their FITC reagents. The expiry dates on those are far longer than any you will get from making your own up. Approximately 12 months +
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Date: Tue, 18 Apr 2006 13:23:28 -0400
From: "Gagnon, Eric"
Subject: [Histonet] Expiry Period - Immunofluorescence Dilutions
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We currently do immunofluorescence for IgG, IgA, IgM, C3, C1q, Fib., Kappa, Lambda, and C4d on renal biopsies, and the first four on skin biopsies. We are using Dako Antibody Diluting Buffer and mostly Dako FITC antisera.
We currently make up about 1 ml of each, and the dilutions last us between 1 and 3 weeks. We would like to keep them longer if possible, making them up in larger quantities, possibly with the goal of transferring them to dispensers on our Ventana XT's.
Is there anyone doing the same immunofluorescence panel and dilutions, and if so, how long are you able to use your dilutions once made?
Thanks for your assistance,
Eric Gagnon MLT
Kingston General Hospital,
Kingston, Ontario, Canada
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