Re: [Histonet] suggestion for sections washing off

From:"John A. Kiernan"

If you put a layer of protein on the slide, you'll
cover up the positively charged glass surface, so
you may as well use ordinary, less expensive
slides. Albumen (from eggs) is a commonly used
adhesive; I don't think albumin is often used for
this purpose.

John Kiernan
Anatomy, UWO
London, Canada.
"Nava, Josefa" wrote:
> Hello to Everyone,
> I am running my IHC using  Ventana  stainer  . Sometimes  a positive
> charged slide is not enough to hold the sections. Any suggestions that I
> can do to the positive  charged slides?
> Will  putting thin coat of Albumin to the positive charged slides do the
> trick? I heard that it  will affect the positivity of the slide. Will it
> not affect the immuno staining?
> Any suggestion is very much appreciated.
> Thanks,
> Josie Nava
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