Re: [Histonet] (no subject)
we cut ours on plus slides and let them air dry. We don't dip them in
acetone. If they are to be stained the next day, we leave them in a
refrigerator overnight. Hope this helps.
Joe Nocito BS, HT(ASCP)QIHC
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
Sent: Wednesday, April 12, 2006 4:29 PM
Subject: [Histonet] (no subject)
> Hi everyone. I have a few questions for you immunofluorescence experts out
> there. I recently have started doing IF on derm cases. I have noticed that
> quite a few of my sections are washing off or folding. So far the doctor
> has been
> able to make a diagnosis on all of the them, but I would like to improve
> methods. After I cut the frozens, I dip the slides in acetone and allow
> to dry completely. I've noticed that the sections start coming off or
> during my PBS washes. I'm very gentle while rinsing but still have
> Any tips would be very much appreciated. Its so frustrating to have
> beautiful sections at the cryostat and end up with very little to show
> for the
> finished product.
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