Re: [Histonet] (no subject)

From:"Joe Nocito"

we cut ours on plus slides and let them air dry. We don't dip them in 
acetone. If they are to be stained the next day, we leave them in a 
refrigerator overnight. Hope this helps.

Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message ----- 
Sent: Wednesday, April 12, 2006 4:29 PM
Subject: [Histonet] (no subject)

> Hi everyone. I have a few questions for you immunofluorescence experts out
> there. I recently have started doing IF on derm cases. I have noticed that
> quite  a few of my sections are washing off or folding. So far the doctor 
> has been
> able  to make a diagnosis on all of the them, but I would like to improve 
> my
> methods.  After I cut the frozens, I dip the slides in acetone and allow 
> them
> to dry  completely. I've noticed that the sections start coming off or 
> folding
> during my  PBS washes. I'm very gentle while rinsing but still have 
> problems.
> Any tips  would be very much appreciated. Its so frustrating to have
> beautiful sections at  the cryostat and end up with very little to show 
> for the
> finished product.
> Thanks!!
> Jodi
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