Re: [Histonet] CK5/6 - nuclear staining
The only time I have observed "nuclear" immunoreactivity with CK5/6 was in a
poorly fixed specimen.
When the aggressive HIER (pressure cooker) was replaced by more gentle
steaming, the reaction disappeared.
This type of artifactual reaction can even rear it's ugly head in well fixed
tissues with some antibodies.
----- Original Message -----
From: "Richard Cartun"
Sent: Friday, April 14, 2006 4:48 PM
Subject: [Histonet] CK5/6 - nuclear staining
> Has anyone observed "nuclear" staining with Dako's cytokeratin 5/6
> monoclonal antibody? I think it may represent an artifact from the heat
> retrieval, but I wanted to query the Histonet membership in case others
> have seen this too. Thank you.
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
> Histonet mailing list
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