RE: [Histonet] cardio myocyte histomorpometry

From:"Elizabeth Chlipala"


I believe most reference just measure the diameter of the myocyte
through the center.  A regular H&E should work.  You can use an ocular
micrometer or take a digital image and use a image analysis program.
That's what we have done in the past.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Patsy
Sent: Wednesday, April 05, 2006 2:47 PM
Subject: [Histonet] cardio myocyte histomorpometry

We want to measure the size of cardio myocytes.  The investigator heard
tell of a "Silver" stain used for this but he doesn't know what silver
stain. What is recommended?  This will be done using a computerized
image analysis system. Thanks in advance, Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
wk email
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