RE: [Histonet] What is your favorite frozen section fixative

From:"Tony Henwood"

Stephen,

We routinely use methanol followed by a rapid H&E. It is important to
immediately fix the slide and not allow any airdrying. 30-60 sec at room
temp is enough. I will forward on to you some example pics on a separate
email

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen
Peters M.D.
Sent: Tuesday, 11 April 2006 12:32 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] What is your favorite frozen section fixative


HI histonetters,
   
  I would like to do a comparison of the morphologic results using
different fixatives
   for frozen section slides. I have always been happy with 95% ETOH. I
know there are other good fixatives people use and I thought it would be
useful to compare them. I will photograph and add these results to my 
  web site tutorial (
http://pathologyinnovations.com/frozen_section_technique.htm ) and
frozen section lecture ( NSH Region 1 April 28 & 29 and California
Society 
  of Histotechnology May 18-21).  
  Please let me know what you find is the best fixative for frozen
section morphology.
   
  Stephen
   
   
   


Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836
 
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 
www.pathologyinnovations.com 




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