RE: [Histonet] Tissue transfer
I'm not familiar with Mount-Quick, but years ago I used to occasionally lift
sections from slides, using a product called Pro-Texx. Here's the protocol I
Remove coverslip from slide by immersion in xylene.
One hour additional time in xylene, to ensure removal of mounting medium.
While slide is damp with xylene, place a drop of Permount on any section
that is to remain on the slide, and a drop of Pro-Texx on the section that
is to be lifted, being careful the two media do not mix. Do not spread the
media thin. You want a thick layer.
Allow to dry thoroughly, using gentle heat (low setting of a slide warmer).
Alternatively they can dry overnight at room temperature.
Immerse slide in distilled water until the Pro-Texx loosens from the slide
(the Permount will not).
With forceps, peel the layer of Pro-Texx, containing the tissue section, off
I did this many years ago and we did not have charged slides at that time,
so I would place the Pro-Texx film, tissue side down, onto a slide smeared
with a thin layer of albumen, lay it flat on the same warm surface, cover it
with a small piece of polyethylene film, and place a flat weight on top of
it to ensure good contact between tissue and slide until dry.
Remove Pro-Texx by immersion in xylene.
Presumably using charged slides might facilitate this final step. If the
process you are doing is similar to this, then obviously you would want the
> From: firstname.lastname@example.org on behalf of
> Lester Raff
> Sent: Thursday, April 13, 2006 12:05 PM
> To: email@example.com
> Subject: [Histonet] Tissue transfer
> I have been reading about a procedure for transferring small fragments
> of tissue from stained slides to charged slides for IHC. The procedure
> calls for Mount-Quick mounting medium. I have found this on the web,
> but don't know whether the procedure replies to the aqueous based or
> solvent based product.
> 1. Have people used a transfer procedure like this effectively?
> 2. Which Mount-Quick is used?
> 3. Would anyone like to share a their protocol?
> Lester J. Raff, MD
> Medical Director
> UroPartners, LLC Laboratory
> ph: 708-486-0076
> fax: 708-486-0080
> Histonet mailing list
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