Hi Gayle,

Yes, you are right.  "PVA" is commonly used in reference to polyvinyl
alcohol, and to polyvinyl acetate as well, so we should be sure which
compound we are talking about.  Interesting that polyvinyl alcohol can be
used as a releasing agent while polyvinyl acetate is an adhesive.  In print
I believe I have seen the designation PVAc in reference to polyvinyl
acetate, but in speech, in my experience, people commonly refer to both
compounds as PVA.

> ----------
> From: 	Gayle Callis
> Sent: 	Monday, April 3, 2006 12:57 PM
> To: 	Monfils, Paul
> Subject: 	RE: [Histonet] Tissue graft coming off of slide  - P.S.
> 
> I always thought PVA stood for poly vinyl alcohol?   AT least it is on the
> 
> OCT cryoembedding media bottle.
> 
> At 01:41 PM 4/3/2006, you wrote:
> >P.S.  I just recalled another method that gave me good results with
> >synthetics, which I still use occasionally, namely PVA (polyvinyl
> acetate)
> >in the water bath, followed by drying as described in my previous post.
> PVA
> >makes the water bath milky white, which makes it more difficult to see
> the
> >sections floating on it - which is why I prefer the chrome alum gelatin -
> >but it is an excellent adhesive for difficult materials.  It can be
> >purchased in the form of "Elmer's Glue-All" or "Sobo Glue" or various
> other
> >brands.  Not all "white glues" are PVA however, so read the product
> label.
> >Some people also use the tan-colored "carpenter's wood glue" which is
> >available in hardware stores.  I haven't used this myself, and am not
> sure
> >of the chemical makeup of the product.
> >
> > > ----------
> > > From:         histonet-bounces@lists.utsouthwestern.edu on behalf of
> > > bob-meyer@northwestern.edu
> > > Reply To:     bob-meyer@northwestern.edu
> > > Sent:         Monday, April 3, 2006 11:46 AM
> > > To:   Histonet@lists.utsouthwestern.edu
> > > Subject:      [Histonet] Tissue graft coming off of slide
> > >
> > >
> > > Just wondering if anyone has any ideas to keep ePTFE (I will ask
> > > researcher what this stands for)
> > > graft tissue from porcine from coming off of slides during antigen
> > > retrieval.  Actually, it is
> > > starting to come off even after deparaffinization.  I told the
> researcher
> > > this is our last hope
> > > that someone on the Histonet might have an idea.  Here is a summary of
> > > what I have tried.  I used
> > > silanized slides (from Dako) with an overnight bake at 57C for tissue
> > > adherance plus an additional
> > > 10 minute 10% NBF soak to fix the tissue firmly on the slide after
> > > deparaffinization and before
> > > antigen retrieval.  I have used low temperature antigen retrieval
> (LTAR)
> > > in the past for delicate
> > > tissue, but if the tissue comes off after deparaffinization what is
> the
> > > point.  Is there something
> > > else out there that I can try?  Thanks for any and all help.
> > >
> > > Bob Meyer
> > > Sr. Research Technologist
> > > Northwestern University
> > > Chicago, IL  60445
> > > 312-908-5546
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet@lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> 
> 
> 

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