Hello,

i have a problem with my toluidine blue staining after radioactive in situ 
hybridization. After developing the slides i stain them with 0,2% 
toluidine blue. After staining I often see under the microscope a red 
background colour on the slide and in the tissue. Therefore I can't see my 
radioactive signal properly. Did anybody have the same problem and can 
help me? I don't know what to do, because it just appears sometimes, even 
when the procedure is the same.

Thanks.

Steffi 

-- 
Analog-/ISDN-Nutzer sparen mit GMX SmartSurfer bis zu 70%!
Kostenlos downloaden: http://www.gmx.net/de/go/smartsurfer

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet