[Histonet] re-freezing frozen sections
I have tissues(monkey brain) that were fixed(4%Paraformaldehyde),
cryprotected(20% sucrose), and flash froze(-70C isopentane). Unfortunately,
mothernature(hurricane katrina) came threw and destroyed everything. This
tissues sat for 3-4 months at blistering temps in the same position(melted OCT)
& some even had a surprise for me(fungus). When the University let us come back
into our building, I rinsed off OCT and put the tissues in 10%NBF at room temp.
The tissue was immersed for 3-4months in fixative. I rinsed the tissue and once
again, flash froze one of the sections, and they cut horribly. I don't know
what to do. I am now tempting to paraffin process. I prefer to do frozens
because its easier to get almost every section of tissue(one sections I usually
get 500-700 slides). As most of you know, brain likes to explode in water, so I
can do a better job if they are frozen.
Any help, I would appreciate!
Dina D. Gaupp, B.S., M.T.
Medical Research Specialist
Center for Gene Therapy
Tulane University Health Sciences Center
JBJ Bldg/Rm 658, SL-99
1430 Tulane Avenue
New Orleans, LA 70112
Histonet mailing list
<< Previous Message | Next Message >>