[Histonet] (no subject)


Hi everyone. I have a few questions for you immunofluorescence experts out  
there. I recently have started doing IF on derm cases. I have noticed that 
quite  a few of my sections are washing off or folding. So far the doctor has been 
able  to make a diagnosis on all of the them, but I would like to improve my 
methods.  After I cut the frozens, I dip the slides in acetone and allow them 
to dry  completely. I've noticed that the sections start coming off or folding 
during my  PBS washes. I'm very gentle while rinsing but still have problems. 
Any tips  would be very much appreciated. Its so frustrating to have 
beautiful sections at  the cryostat and end up with very little to show for the 
finished product.
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