[Histonet] liver damage detection
I am working on an experimental model of viral liver damage. Does
anyone have suggestions as to how I should best proceed in
establishing the type or extent of damage caused? I don't expect
damage to the extent that the mice die or even show any overt signs
of illness. There is no noticeable gross change in the liver.
Ideally I would like to establish a biochemical marker of damage such
as serum ALT as well as a morphological analysis. I figure an H&E
stain would be enough. I would like to use NBF fixed tissue. Are
there any suggestions as to the thickness of the liver sections? I
can reliably section 30 um, anything thinner would require special
Any suggestions for a kit to measure ALT? A colleague suggested that
I submit the samples to the hospital's diagnostic lab, but would they
be able to detect mouse ALT?
Any suggestions would be appreciated. I have never worked with liver
damage, so I really don't know what to do here.
Thanks in advance,
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